A standardized protocol for the determination of specific leaf area and leaf dry matter content
A standardized protocol for the determination of specific leaf area and leaf dry matter content
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DOI：
10.1046/j.0269-8463.2001.00563.x
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发表时间：
2001-10-01
影响因子：
5.2
通讯作者：
Laurent, G
中科院分区：
文献类型：
--
作者：
Garnier, E;Shipley, B;Laurent, G
1.以田间生长的三种野生植物为材料，研究了样品制备、复水程序和采集时间对成熟叶片比叶面积(SLA)和叶干物质含量(LDMC)测定的影响。样品在放入水中6小时后即可完全复水，但所测试的两种程序--复水前的样品制备或复水过程中的温度--都不会对SLA或LDMC.3的最终值产生显著影响。正如预期的那样，水饱和的叶片的LDMC低于未复水的叶片；更令人惊讶的是，它们的SLA也更高。当物种的SLA较高时，复水对SLA的影响尤为重要。在所覆盖的时间段(09.00-16.30h)内，采样时间对任何物种的这两个性状都没有显著影响。这些结果表明，在水分饱和叶片上获得的SLA和LDMC(SLA(SAT)和LDMCSAT)可以用于物种比较。我们提出了一种测量这些特征的标准化方案。这将使数据收集更好地保持一致性，这是建立大型功能特征数据库的先决条件。
1. The impact of sample preparation, rehydration procedure and time of collection on the determination of specific leaf area (SLA, the ratio of leaf area to leaf dry mass) and leaf dry matter content (LDMC, the ratio of leaf dry mass to fresh mass) of mature leaves was studied in three wild species growing in the field, chosen for their contrasting SLA and LDMC.2. Complete rehydration was achieved 6 h after samples were placed into water, but neither of the procedures tested-preparation of samples before rehydration or temperature applied during rehydration-had a significant effect on the final values of SLA or LDMC.3. As expected, water-saturated leaves had a lower LDMC than non-rehydrated leaves; more surprisingly, their SLA was also higher. The impact of rehydration on SLA was especially important when the SLA of the species was high.4. There was no significant effect of time of sampling on either trait in any species over the time period covered (09.00-16.30 h).5. These results suggest that SLA and LDMC obtained on water-saturated leaves (SLA(SAT) and LDMCSAT) can be used for species comparisons. We propose a standardized protocol for the measurement of these traits. This would allow for better consistency in data collection, a prerequisite for the constitution of large databases of functional traits.