Identification of multiple genetic loci in the mouse controlling immobility time in the tail suspension and forced swimming tests
Identification of multiple genetic loci in the mouse controlling immobility time in the tail suspension and forced swimming tests
复制标题
DOI：
10.14943/jjvr.63.2.53
复制
发表时间：
2015-05-01
影响因子：
0.4
通讯作者：
Agui, Takashi
中科院分区：
文献类型：
--
作者：
Abou-Elnaga, Ahmed F.;Torigoe, Daisuke;Agui, Takashi
抑郁症是世界各国最著名的人类精神疾病之一，被认为是一种复杂的神经行为特征，难以识别致病基因。悬尾试验（TST）和强迫游泳试验（FST）被广泛用于评价小鼠的抑郁样行为和抗抑郁活性。已知多种抗抑郁药可减少TST和FST中的不动时间。为了确定两种试验中不动持续时间的遗传决定因素，我们分析了101只来自C57 BL/6和DBA/2品系间杂交的F-2小鼠。利用106个微卫星标记进行的数量性状基因座（QTL）定位显示，TST和FST中分别有3个控制不动时间的基因座（2个显著基因座和1个提示基因座）和5个提示基因座。QTL分析的结果表明，抑郁症的遗传结构的广泛描述，确定新的抗抑郁药的分子靶点，以清除抑郁症的复杂遗传机制提供了基础。
Depression is one of the most famous psychiatric disorders in humans in all over the countries and considered a complex neurobehavioral trait and difficult to identify causal genes. Tail suspension test (TST) and forced swimming test (FST) are widely used for assessing depression-like behavior and antidepressant activity in mice. A variety of antidepressant agents are known to reduce immobility time in both TST and FST. To identify genetic determinants of immobility duration in both tests, we analyzed 101 F-2 mice from an intercross between C57BL/6 and DBA/2 strains. Quantitative trait locus (QTL) mapping using 106 microsatellite markers revealed three loci (two significant and one suggestive) and five suggestive loci controlling immobility time in the TST and FST, respectively. Results of QTL analysis suggest a broad description of the genetic architecture underlying depression, providing underpinnings for identifying novel molecular targets for antidepressants to clear the complex genetic mechanisms of depressive disorders.