Cardiac fibroblasts: function, regulation of gene expression, and phenotypic modulation.
Cardiac fibroblasts: function, regulation of gene expression, and phenotypic modulation.
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DOI：
10.1007/978-3-642-72477-0_16
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发表时间：
1992
影响因子：
9.5
通讯作者：
M. Eghbali
中科院分区：
文献类型：
--
作者：
M. Eghbali
心脏成纤维细胞构成心室肌中的大多数非肌细胞细胞群体。这些细胞位于心肌细胞之间和周围的区域。心脏成纤维细胞负责合成细胞外基质蛋白，如纤维状胶原I型和III型、基底膜IV型胶原、纤连蛋白和层粘连蛋白。除了其在肌肉发育和成肌细胞分化中的作用之外，主要由纤维状胶原组成的细胞外基质是一种复杂且高度组织化的结构，其用于支持心肌细胞并维持心肌的功能完整性。这种基质的平衡合成和降解是心肌正常发育和完善心肌功能的关键。胶原重塑和积聚已在几种心脏肥大的实验模型中得到证实。为了深入了解影响心脏成纤维细胞行为的分子和细胞机制，培养了大鼠和兔心室肌的心脏成纤维细胞，并研究了心脏成纤维细胞体内暴露的神经递质和生长因子（如去甲肾上腺素和转化生长因子-β（TGF-β1））的影响。这些研究的结果，关于基因表达，增殖和分化的心脏成纤维细胞在文化中，其生物学意义进行了讨论。
Cardiac fibroblasts constitute the majority of the non-myocyte cell population in the ventricular myocardium. These cells are located in the interstitium, in areas between and surrounding cardiac myocytes. Cardiac fibroblasts are responsible for the synthesis of extracellular matrix proteins such as fibrillar collagen types I and III, basement membrane type IV collagen, fibronectin, and laminin. In addition to its role in muscle development and myoblast differentiations, extracellular matrix consisting primarily of fibrillar collagen is an intricate and highly organized structure that serves to support cardiac myocytes and to maintain functional integrity of the myocardium. Balanced synthesis and degradation of this matrix is the key to normal development of cardiac muscle and perfect myocardial function. Collagen remodeling and accumulation has been demonstrated in several experimental models of cardiac hypertrophy. To gain insights into molecular and cellular mechanisms that affect cardiac fibroblast behavior, cardiac fibroblasts from rat and rabbit ventricular myocardium were cultured and the impact of neurotransmitters and growth factors such as norepinephrine and transforming growth factor — beta (TGF-β1), to which cardiac fibroblasts are exposed in vivo, was studied. Results of these studies, with regards to gene expression, proliferation and differentiation of cardiac fibroblasts in culture, and their biological implications are discussed.