Human skeletal muscle nitrate store: influence of dietary nitrate supplementation and exercise.
Human skeletal muscle nitrate store: influence of dietary nitrate supplementation and exercise.
复制标题
人体骨骼肌硝酸盐储存：饮食中补充硝酸盐和运动的影响。
DOI：
10.1113/jp278076
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发表时间：
2019-12
影响因子：
5.5
通讯作者：
Piknova, Barbora
中科院分区：
文献类型：
--
作者：
Wylie, Lee J.;Park, Ji Won;Vanhatalo, Anni;Kadach, Stefan;Black, Matthew, I;Stoyanov, Zdravko;Schechter, Alan N.;Jones, Andrew M.;Piknova, Barbora
啮齿动物骨骼肌含有大量的硝酸盐，可以通过摄入食物中的硝酸盐来增加。这个肌肉硝酸盐储存库被发现是亚硝酸盐和一氧化氮(NO)的重要来源，它是被组织黄嘌呤氧化还原酶还原的。为了探索这一途径在运动过程中是否也在人类骨骼肌中活跃，以及它是否对局部硝酸盐的可获得性敏感，我们评估了运动诱导的年轻健康人肌肉硝酸盐和亚硝酸盐浓度的变化，在基线条件下和饮食硝酸盐摄入之后。我们发现，肌肉中硝酸盐和亚硝酸盐的基线浓度远远高于血浆中的硝酸盐和亚硝酸盐浓度(分别为~4倍和~29倍)，并且单剂膳食硝酸盐(12.8 mmol)的摄入显著增加了血浆(~19倍)和肌肉(~5倍)的硝酸盐浓度。与这些观察结果一致，并与先前关于肌肉硝酸盐转运活跃的建议一致，我们提出的蛋白质印迹数据显示，活跃的硝酸盐/亚硝酸盐转运体sialin在人类骨骼肌中显著表达。此外，我们报告了运动引起的人体肌肉硝酸盐浓度的降低(~39%)，但只有在肌肉硝酸盐储存增加的情况下才会如此。我们的结果表明，人体骨骼肌中的硝酸盐储备对饮食中的硝酸盐摄入量很敏感，这可能是运动中产生NO的原因之一。综上所述，这些发现表明，骨骼肌在人体硝酸盐的运输、储存和新陈代谢中发挥着重要作用。
Rodent skeletal muscle contains a large store of nitrate that can be augmented by the consumption of dietary nitrate. This muscle nitrate reservoir has been found to be an important source of nitrite and nitric oxide (NO) via its reduction by tissue xanthine oxidoreductase. To explore if this pathway is also active in human skeletal muscle during exercise, and if it is sensitive to local nitrate availability, we assessed exercise-induced changes in muscle nitrate and nitrite concentrations in young healthy humans, under baseline conditions and following dietary nitrate consumption. We found that baseline nitrate and nitrite concentrations were far higher in muscle than in plasma (~4-fold and ~29-fold, respectively), and that the consumption of a single bolus of dietary nitrate (12.8 mmol) significantly elevated nitrate concentration in both plasma (~19-fold) and muscle (~5-fold). Consistent with these observations, and with previous suggestions of active muscle nitrate transport, we present western blot data to show significant expression of the active nitrate/nitrite transporter sialin in human skeletal muscle. Furthermore, we report an exercise-induced reduction in human muscle nitrate concentration (by ~39%), but only in the presence of an increased muscle nitrate store. Our results indicate that human skeletal muscle nitrate stores are sensitive to dietary nitrate intake and may contribute to NO generation during exercise. Together, these findings suggest that skeletal muscle plays an important role in the transport, storage and metabolism of nitrate in humans.