Proteomic Analysis of Plasmodium Merosomes: The Link between Liver and Blood Stages in Malaria.
Proteomic Analysis of Plasmodium Merosomes: The Link between Liver and Blood Stages in Malaria.
复制标题
疟原虫粒体的蛋白质组学分析：疟疾肝脏阶段和血液阶段之间的联系。
DOI：
10.1021/acs.jproteome.9b00324
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发表时间：
2019
影响因子：
4.4
通讯作者：
Sinnis,Photini
中科院分区：
文献类型：
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作者：
Shears,MelanieJ;SekharNirujogi,Raja;Swearingen,KristianE;Renuse,Santosh;Mishra,Satish;JaipalReddy,Panga;Moritz,RobertL;Pandey,Akhilesh;Sinnis,Photini
疟疾寄生虫的红细胞前肝脏阶段，包括子孢子及其发育成的肝脏阶段，仍然是生命周期中了解最少的部分之一，部分原因是寄生虫数量很少。尽管如此，它仍被认为是抗疟疾药物和疫苗的重要目标。在这里，我们提供了第一个蛋白质组分析，它定义了肝脏阶段的最后阶段，并负责启动血液阶段的感染。我们总共鉴定了1879种寄生虫蛋白质，并在每个生物复制中定量检测到1188种蛋白质的核心集合，提供了这一阶段蛋白质谱的广泛图景。这一独特的数据集将使我们能够探索关于分生体和肝脏裂殖子生物学的关键问题。
The pre-erythrocytic liver stage of the malaria parasite, comprising sporozoites and the liver stages into which they develop, remains one of the least understood parts of the lifecycle, in part owing to the low numbers of parasites. Nonetheless, it is recognized as an important target for antimalarial drugs and vaccines. Here we provide the first proteomic analysis of merosomes, which define the final phase of the liver stage and are responsible for initiating the blood stage of infection. We identify a total of 1879 parasite proteins, and a core set of 1188 proteins quantitatively detected in every biological replicate, providing an extensive picture of the protein repertoire of this stage. This unique data set will allow us to explore key questions about the biology of merosomes and hepatic merozoites.