Effect of praziquantel on adult Echinococcus granulosus in vitro: scanning electron microscopy.
Effect of praziquantel on adult Echinococcus granulosus in vitro: scanning electron microscopy.
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吡喹酮对体外细粒棘球绦虫成虫的影响：扫描电子显微镜。
DOI：
10.1007/bf00925726
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
Andersen,FL
中科院分区：
文献类型：
--
作者：
Conder,GA;Marchiondo,AA;Andersen,FL
与对照相比，吡喹酮在体外浓度为 5、50 和 500 ppm 1 小时的作用导致细粒棘球绦虫成虫的皮膜进行性破坏和形态扭曲。对用不同浓度的吡喹酮处理的所有标本进行扫描电子显微镜观察，结果显示，即使不是全部，也大部分的喙钩消失，吸盘结构发生变化。许多绦虫在被放入药物后立即从宿主的肠道中脱离，并且所有经过治疗的绦虫都表现出收缩或肿胀，特别是在倒数第二个节片中。暴露于较高药物浓度的线虫明显出现强烈收缩。正如在对照标本中观察到的，当暴露于 5 ppm 的吡喹酮时，末端节片上的特征性圆锥形微毛变得融合和杂乱。在药物浓度为 50 ppm 时，外皮表面在融合的微毛团之间形成凹槽或沟渠，而 500 ppm 则导致寄生虫的裸露外皮内产生孔洞。在浓度为 50 ppm 的绦虫的外表面和皮膜下方观察到卵形体，推测为卵。这些结构似乎还粘附在暴露于 500 ppm 浓度的样品的外表面。鉴于上述情况，在处理和处置吡喹酮治疗后受感染或可疑犬的粪便时应特别小心，因为大肠杆菌的皮膜表面会破裂。颗粒细胞可能会导致潜在感染性虫卵的释放。
The effect of praziquantel in vitro at concentrations of 5, 50 and 500 ppm for 1 h resulted in the progressive breakdown of the tegument and in morphologic distortion of adultEchinococcus granulosuswhen compared to controls. Scanning electron microscopy of all specimens treated in the various concentrations of praziquantel showed loss of most, if not all, of the rostellar hooks and changes in the structure of the suckers. Many of the tapeworms immediately detached from the host's gut upon being placed in the drug, and all treated cestodes exhibited contraction or swelling, particularly in the penultimate proglottid. Intense contraction was apparent in the worms exposed to the higher drug concentrations. Characteristic conical microtriches on the terminal proglottid, as observed in the control specimens, became fused and matted when exposed to 5 ppm of praziquantel. At a drug concentration of 50 ppm, the tegumental surface developed grooves or furrows between clumps of fused microtriches, while 500 ppm caused production of holes within the denuded tegument of the parasite. Ovoid bodies, presumed to be eggs, were observed on the outer surfaces and just below the tegument of tapeworms treated with concentrations of 50 ppm. These structures also appeared to adhere to the outer surfaces of specimens exposed to 500 ppm. In view of the foregoing, special care should be taken in handling and disposing of feces from infected or suspect dogs after praziquantel treatment, since the breakdown in the tegumental surface ofE. granulosuspresumably results in the release of potentially infective eggs.