Cell-extracellular matrix versus cell-cell interactions during the development of the cochlear-vestibular ganglion.
Cell-extracellular matrix versus cell-cell interactions during the development of the cochlear-vestibular ganglion.
复制标题
耳蜗前庭神经节发育过程中细胞-细胞外基质与细胞-细胞相互作用。
DOI：
10.1002/jnr.22664
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发表时间：
2011
影响因子：
4.2
通讯作者：
Davies D
中科院分区：
文献类型：
--
作者：
Davies D
注定成为耳蜗前庭神经节（CVG）的神经元的细胞起源于耳上皮内。在发育早期，它们与相邻细胞分离并迁移出上皮，在那里它们合并形成CVG。为了完成这一过程，神经母细胞必须改变它们与上皮内其他细胞和细胞外基质中蛋白质的相互作用，以允许重新定位。本研究的目的是调查的主要家族的粘附分子介导的细胞与细胞外基质，整合素，并与其他细胞，钙粘蛋白，在成神经细胞分离耳上皮细胞的相互作用的作用。经典的钙粘蛋白的表达增加迁移成神经细胞相比，耳上皮。定量RT-PCR显示，这与迁移细胞中E-钙粘蛋白的下调和N-钙粘蛋白的上调同时发生。相反，在迁移的成神经细胞中，上皮细胞的β1整合素表达水平得以维持。然而，虽然多个整合素配体表达在耳基底膜在这个阶段的发展，只有纤连蛋白（FN）支持成神经细胞迁移沿着基板在体外。β1整合素的抑制导致FN上的线性迁移显著减少。重要的是，神经母细胞保留了从上皮分离的能力，但仍然紧密靠近原始组织，这表明细胞-细胞之间的相互作用优于细胞-基质之间的相互作用。这些数据表明，细胞-细胞和细胞-基质之间的相互作用的平衡指导耳成神经细胞迁移和神经节。© 2011 Wiley利斯公司
Cells destined to become the neurones of the cochlear‐vestibular ganglion (CVG) originate within the otic epithelium. Early in development they detach from their neighbors and migrate out of the epithelium, where they coalesce to form the CVG. To accomplish this process, the neuroblasts must modify their interactions with other cells within the epithelium and with proteins in the extracellular matrix to allow for repositioning. The aim of this study was to investigate the roles of the major families of adhesion molecules that mediate cellular interactions with the extracellular matrix, the integrins, and with other cells, the cadherins, in neuroblast segregation from the otic epithelium. The expression of classical cadherins increased in migrating neuroblasts compared with the otic epithelium. Quantitative RT‐PCR revealed that this was concomitant with down‐regulation of E‐cadherin and up‐regulation of N‐cadherin in the migrating cells. In contrast, the level of β1 integrin expression by the epithelium was maintained in migrating neuroblasts. However, although multiple integrin ligands were expressed within the otic basement membrane at this stage of development, only fibronectin (FN) supported neuroblast migration along the substrate in vitro. Inhibition of β1 integrins resulted in significantly reduced linear migration on FN. Importantly, neuroblasts retained the ability to segregate from the epithelium but remained compacted immediately adjacent to the originating tissue, suggesting dominance of cell–cell over cell–matrix interactions. These data suggest that the balance between cell–cell and cell–substratum interactions directs otic neuroblast migration and gangliogenesis. © 2011 Wiley‐Liss, Inc.