Human mammary luminal epithelial cells contain progenitors to myoepithelial cells
Human mammary luminal epithelial cells contain progenitors to myoepithelial cells
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DOI：
10.1006/dbio.1998.9133
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发表时间：
1999-02-01
影响因子：
2.7
通讯作者：
Petersen, OW
中科院分区：
文献类型：
--
作者：
Péchoux, C;Gudjonsson, T;Petersen, OW
人乳腺上皮细胞和肌上皮细胞的起源尚未阐明。在这项研究中，我们已经解决了腔上皮细胞和肌上皮细胞是否垂直连接，即，一个是另一个的先驱。我们使用了原代培养试验，允许在培养中保存管腔上皮细胞和肌上皮细胞的基本表型性状。然后使用针对谱系特异性细胞表面抗原的抗体免疫磁性分离这两种细胞类型至最高100%纯度。通过细胞化学、免疫印迹和2-D凝胶电泳确定细胞身份。腔上皮细胞通过细胞角蛋白18和19的强表达来鉴定，而肌上皮细胞通过波形蛋白和α-平滑肌肌动蛋白的表达来识别。我们使用了先前设计的允许增殖性肌上皮细胞剧烈扩增的培养基（CDM 4），并且还设计了允许基于添加肝细胞生长因子/分散因子的分化的腔上皮细胞充分扩增的培养基（CDM 6）。两种不同的培养基支持每种谱系至少5次传代，没有相互转化的迹象。我们使用平行培养，其中我们切换培养基，从而测试每个谱系转化为另一个谱系的能力。而肌上皮谱系没有表现出相互转化的迹象，一个子集的腔上皮细胞，逐渐地，但明显地，转化为肌上皮细胞。我们认为，在成熟的人乳腺中，是腔上皮细胞室产生肌上皮细胞，而不是相反。(C)北京：科学出版社.
The origin of the epithelial and myoepithelial cells in the human breast has not been delineated. In this study we have addressed whether luminal epithelial cells and myoepithelial cells are vertically connected, i.e., whether one is the precursor for the other. We used a primary culture assay allowing preservation of basic phenotypic traits of luminal epithelial and myoepithelial cells in culture. The two cell types were then separated immunomagnetically using antibodies directed against lineage-specific cell surface antigens into at best 100% purity. The cellular identity was ascertained by cytochemistry, immunoblotting, and 2-D gel electrophoresis. Luminal epithelial cells were identified by strong expression of cytokeratins 18 and 19 while myoepithelial cells were recognized by expression of vimentin and alpha-smooth muscle actin. We used a previously devised culture medium (CDM4) that allows vigorous expansion of proliferative myoepithelial cells and also devised a medium (CDM6) that allowed sufficient expansion of differentiated luminal epithelial cells based on addition of hepatocyte growth factor/scatter factor. The two different culture media supported each lineage for at least five passages without signs of interconversion. We used parallel cultures where we switched culture media, thus testing the ability of each lineage to convert to the other. Whereas the myoepithelial lineage showed no signs of interconversion, a subset of luminal epithelial cells, gradually, but distinctly, converted to myoepithelial cells. We propose that in the mature human breast, it is the luminal epithelial cell compartment that gives rise to myoepithelial cells rather than the other way around. (C) 1999 Academic Press.