Particle tracking in drug and gene delivery research: State-of-the-art applications and methods.
Particle tracking in drug and gene delivery research: State-of-the-art applications and methods.
复制标题
药物和基因递送研究中的粒子跟踪：最新的应用和方法。
DOI：
10.1016/j.addr.2015.03.017
复制
发表时间：
2015-08-30
影响因子：
16.1
通讯作者：
Hanes J
中科院分区：
文献类型：
--
作者：
Schuster BS;Ensign LM;Allan DB;Suk JS;Hanes J
粒子跟踪是一种强大的显微镜技术，用于在复杂流体和生物标本中以高空间和时间分辨率量化单个粒子的运动。粒子跟踪在药物和基因传递研究中的应用和影响在过去十年中大大增加。由于硬件和软件的进步，这项技术现在比以往任何时候都更容易获得，并且可以可靠地自动化以实现大型数据集的快速处理，从而进一步增强粒子跟踪在未来药物和基因传递研究中的作用。我们首先讨论基于颗粒跟踪的进展，以表征治疗性纳米颗粒的细胞外和细胞屏障，以及表征纳米颗粒的大小和稳定性。为了促进更广泛地采用该技术，我们提出了一个用户友好的最先进的自动粒子跟踪算法和分析方法的回顾。最后，我们回顾了下一代粒子跟踪方法的技术发展，并展望了粒子跟踪在药物和基因传递方面的未来研究方向。
Particle tracking is a powerful microscopy technique to quantify the motion of individual particles at high spatial and temporal resolution in complex fluids and biological specimens. Particle tracking's applications and impact in drug and gene delivery research have greatly increased during the last decade. Thanks to advances in hardware and software, this technique is now more accessible than ever, and can be reliably automated to enable rapid processing of large data sets, thereby further enhancing the role that particle tracking will play in drug and gene delivery studies in the future. We begin this review by discussing particle tracking-based advances in characterizing extracellular and cellular barriers to therapeutic nanoparticles and in characterizing nanoparticle size and stability. To facilitate wider adoption of the technique, we then present a user-friendly review of state-of-the-art automated particle tracking algorithms and methods of analysis. We conclude by reviewing technological developments for next-generation particle tracking methods, and we survey future research directions in drug and gene delivery where particle tracking may be useful.