Preparation of a heat-shock protein 70-based vaccine from DC-tumor fusion cells.
Preparation of a heat-shock protein 70-based vaccine from DC-tumor fusion cells.
复制标题
从 DC 肿瘤融合细胞中制备基于热休克蛋白 70 的疫苗。
DOI：
10.1007/978-1-61779-295-3_19
复制
发表时间：
2011
期刊：
影响因子：
--
通讯作者：
Gong,Jianlin
中科院分区：
文献类型：
--
作者：
Weng,Desheng;Calderwood,StuartK;Gong,Jianlin
我们通过在肿瘤和树突状细胞（DC）融合后快速分离热休克蛋白70肽复合物（Hsp70.PC）（Hsp70.PC-F），开发了一种基于增强型分子伴侣的疫苗。在这种方法中，通过细胞融合过程将肿瘤抗原引入树突状细胞的抗原加工机器中，从而获得经过树突状细胞加工的抗原性肿瘤肽或其中间体。我们的结果表明，与单独从肿瘤细胞制备的制剂相比，Hsp70.PC-F 具有增加的免疫原性，因此，构成了基于伴侣蛋白的肿瘤疫苗的改进制剂。
We have developed an enhanced molecular chaperone-based vaccine through rapid isolation of heat-shock protein 70 peptide complexes (Hsp70.PC) after the fusion of tumor and dendritic cells (DCs) (Hsp70.PC-F). In this approach, the tumor antigens are introduced into the antigen-processing machinery of dendritic cells through the cell fusion process and, thus, we can obtain antigenic tumor peptides or their intermediates that have been processed by dendritic cells. Our results show that Hsp70.PC-F has increased immunogenicity compared to preparations from tumor cells alone and, therefore, constitutes an improved formulation of chaperone protein-based tumor vaccine.