A molecular linkage map with associated QTLs from a hulless x covered spring oat population
A molecular linkage map with associated QTLs from a hulless x covered spring oat population
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DOI：
10.1007/s00122-003-1556-x
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发表时间：
2004-05-01
影响因子：
5.4
通讯作者：
McElroy, AR
中科院分区：
文献类型：
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作者：
De Koeyer, DL;Tinker, NA;McElroy, AR
在春型燕麦（Avena sativa L.）中，在适应群体中检测到的数量性状位点（QTL）可能具有通过标记辅助选择改良种质的最大潜力。F-6重组自交系（RI）群体是由两个加拿大春燕麦品种（'Terra'，一个无壳系，和'Marion'，一个精英覆盖种子系）之间的杂交发展而来的。利用430个AFLP、RFLP、RAPD、SCAR和表型标记对101个RI系进行了分子连锁图谱的构建。该图谱通过选择映射到35个连锁群并包含35个非连锁基因座的124个框架标记的稳健集合来细化。1992年至1997年期间，在加拿大和美国的13个田间环境中生长的101个品系进行了16个农艺性状、籽粒性状和化学成分性状的评价。QTL定位使用三种检测方法与实验范围内的错误率约为0.05，每个性状。共检测到34个主效QTL，影响以下性状：抽穗期、株高、倒伏、视觉评分、籽粒产量、籽粒重、碾磨产量、容重、薄仁和饱满仁、去壳β-葡聚糖浓度、油分浓度和蛋白质。其中几个对应于其他燕麦QTL研究中报道的同源或同源区域中的QTL。还检测到24个QTL与环境的互作和3个上位性互作。控制覆盖/无壳性状（N1）的位点影响了本研究中测量的大多数性状。以N1为协变量的加性QTL模型上级优于基于单独覆盖和无壳亚群体的模型。这种方法也适用于其它主基因分离群体。在这项研究中确定的标记性状协会有相当大的潜力，用于标记辅助选择策略，以改善春燕麦育种计划中的性状。
In spring-type oat (Avena sativa L.), quantitative trait loci (QTLs) detected in adapted populations may have the greatest potential for improving germplasm via marker-assisted selection. An F-6 recombinant inbred (RI) population was developed from a cross between two Canadian spring oat varieties: 'Terra', a hulless line, and 'Marion', an elite covered-seeded line. A molecular linkage map was generated using 430 AFLP, RFLP, RAPD, SCAR, and phenotypic markers scored on 101 RI lines. This map was refined by selecting a robust set of 124 framework markers that mapped to 35 linkage groups and contained 35 unlinked loci. One hundred one lines grown in up to 13 field environments in Canada and the United States between 1992 and 1997 were evaluated for 16 agronomic, kernel, and chemical composition traits. QTLs were localized using three detection methods with an experiment-wide error rate of approximately 0.05 for each trait. In total, 34 main-effect QTLs affecting the following traits were identified: heading date, plant height, lodging, visual score, grain yield, kernel weight, milling yield, test weight, thin and plump kernels, groat beta-glucan concentration, oil concentration, and protein. Several of these correspond to QTLs in homologous or homoeologous regions reported in other oat QTL studies. Twenty-four QTL-by-environment interactions and three epistatic interactions were also detected. The locus controlling the covered/hulless character (N1) affected most of the traits measured in this study. Additive QTL models with N1 as a covariate were superior to models based on separate covered and hulless sub-populations. This approach is recommended for other populations segregating for major genes. Marker-trait associations identified in this study have considerable potential for use in marker-assisted selection strategies to improve traits within spring oat breeding programs.