Amelogenin X impacts age-dependent increase of frequency and number in labial incisor grooves in C57BL/6
Amelogenin X impacts age-dependent increase of frequency and number in labial incisor grooves in C57BL/6
复制标题
牙釉蛋白 X 影响 C57BL/6 中唇切牙凹槽频率和数量的年龄依赖性增加
DOI：
10.1016/j.bbrc.2018.01.073
复制
发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Kondo T.
中科院分区：
文献类型：
--
作者：
Ota MS;Kondo K;Li Y;Iseki S;Yamashita A;Gibson CW;Kondo T.
在几种野生型啮齿动物中，已有上颌门牙上唇沟的报道。以前的研究已经报道了年龄相关的唇沟在C57BL/6小鼠中的中等发生率；然而，对这种沟的发生知之甚少。在本研究中，我们观察了出生后26个月的C57BL/6小鼠的齿沟形成，发现不仅切牙沟槽的出现频率和沟槽的数量都随着年龄的增加而增加。我们通过对12个月大(12个月)和24个月大(24个月)小鼠门牙进行DNA微阵列分析，研究了与年龄相关的沟槽形成的分子机制。Amelx编码主要的釉质基质蛋白amelgenin，被鉴定为12M特异基因。与野生型小鼠相比，Amelx−/−突变体的上颌门牙出现唇沟的频率和数量增加。这些发现表明，Amelx基因影响C57BL/6小鼠唇切牙沟的年龄相关性外观。
Labial grooves in maxillary incisors have been reported in several wild-type rodent species. Previous studies have reported age-dependent labial grooves occur in moderate prevalence in C57BL/6 mice; however, very little is known about the occurrence of such grooves. In the present study, we observed age-dependent groove formation in C57BL/6 mice up to 26 months after birth and found that not only the frequency of the appearance of incisor grooves but also the number of grooves increased in an age-dependent manner. We examined the molecular mechanisms of age-dependent groove formation by performing DNA microarray analysis of the incisors of 12-month-old (12M) and 24-month-old (24M) mice. Amelx, encoding the major enamel matrix protein AMELOGENIN, was identified as a 12M-specific gene. Comparing with wild-type mice, the maxillary incisors of Amelx−/−mutants indicated the increase of the frequency and number of labial grooves. These findings suggested that the Amelx gene impacts the age-dependent appearance of the labial incisor groove in C57BL/6 mice.