Determination of monoamine neurotransmitters in human urine by carrier-mediated liquid-phase microextraction based on solidification of stripping phase
Determination of monoamine neurotransmitters in human urine by carrier-mediated liquid-phase microextraction based on solidification of stripping phase
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基于溶出相固化的载体介导液相微萃取测定人尿液中单胺类神经递质
DOI：
10.1016/j.talanta.2015.06.068
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发表时间：
2015-11-01
期刊：
影响因子：
6.1
通讯作者：
Chen, Bo
中科院分区：
文献类型：
--
作者：
Jiang, Liwei;Chen, Yibang;Chen, Bo
建立了一种基于固相萃取-高效液相色谱-电化学检测器（HPLC-ECD）的载体介导液相微萃取（CM-LPME-SSP）分析尿中单胺类神经递质（MNTs）的新方法。通过在有机相中加入合适的载体，实现了亲水性分析物MNT的同时萃取，具有高富集因子（22.6-36.1倍）和良好的样品净化。开发了一种新的策略，即在反萃取过程中固化水反萃取相，以便于收集小至几微升的反萃取相。结合HPLC-ECD分析，NE、E、DA和5-HT的线性范围分别为0.015-2.0 μ g/mL和0.020-2.0 μ g/mL。检测限和定量限分别为5.5-10.8 ng/mL和15-20 ng/mL。相对回收率在87%~ 108%之间，日内和日间相对标准偏差均低于13%。该方法已成功应用于真实的尿液中MNTs的分析。(C)2015爱思唯尔B. V.保留所有权利。
A novel method was developed for the analysis of monoamine neurotransmitters (MNTs) in human urine by carrier-mediated liquid-phase microextraction based on solidification of stripping phase method (CM-LPME-SSP) coupled with high performance liquid chromatography-electrochemical detector (HPLC-ECD). By adding an appropriate carrier in organic phase, simultaneous extraction of hydrophilic analytes, MNTs, with high enrichment factors (22.6-36.1 folds) and excellent sample cleanup was achieved. A new strategy, solidifying the aqueous stripping phase in the back-extraction process, was developed to facilitate the collection of the stripping phase as small as a few microliters. Combined with HPLC-ECD analysis, the linear ranges of the established method were 0.015-2.0 mu g/mL for NE, E, DA, and 0.020-2.0 mu g/mL for 5-HT. The limits of detection and quantification were in the range of 5.5-10.8 ng/mL and 15-20 ng/mL, respectively. The relative recoveries were in the range of 87-108%, with intraday and interday relative standard deviations lower than 13%. This method was successfully applied to analysis of MNTs in real urine. (C) 2015 Elsevier B.V. All rights reserved.