Genome-Wide Identification and Expression Pattern Analysis of Dirigent Members in the Genus Oryza.
Genome-Wide Identification and Expression Pattern Analysis of Dirigent Members in the Genus Oryza.
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DOI：
10.3390/ijms24087189
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发表时间：
2023-04-13
影响因子：
5.6
通讯作者：
Liang, Yun-Kuan
中科院分区：
文献类型：
--
作者：
Duan, Wen;Xue, Baoping;He, Yaqian;Liao, Shenghao;Li, Xuemei;Li, Xueying;Liang, Yun-Kuan
Dirigent （DIR）成员在植物生长发育和适应环境变化中发挥着重要作用。然而，到目前为止，还没有对水稻属的DIR成员进行系统的分析。本研究从9个水稻品种中鉴定出420个具有保守DIR结构域的基因。重要的是，栽培稻比野生稻具有更多的DIR家族成员。根据系统发育分析，水稻DIR蛋白可分为6个亚科。基因重复事件分析表明，全基因组/片段重复和串联重复是水稻DIR基因进化的主要驱动因素，而串联重复是DIR-b/d和DIR-c亚家族基因扩增的主要机制。RNA测序数据分析表明，OsjDIR基因响应广泛的环境因子，大多数OsjDIR基因在根中具有高表达水平。定性反转录PCR分析证实了OsjDIR基因对矿质元素供应不足、重金属过剩和枯丝核菌感染的响应性。此外，DIR家族成员之间存在广泛的相互作用。综上所述，我们的研究结果为进一步探索水稻DIR基因提供了研究基础。
Dirigent (DIR) members have been shown to play essential roles in plant growth, development and adaptation to environmental changes. However, to date, there has been no systematic analysis of the DIR members in the genus Oryza. Here, 420 genes were identified from nine rice species to have the conserved DIR domain. Importantly, the cultivated rice species Oryza sativa has more DIR family members than the wild rice species. DIR proteins in rice could be classified into six subfamilies based on phylogeny analysis. Gene duplication event analysis suggests that whole genome/segmental duplication and tandem duplication are the primary drivers for DIR genes’ evolution in Oryza, while tandem duplication is the main mechanism of gene family expansion in the DIR-b/d and DIR-c subfamilies. Analysis of the RNA sequencing data indicates that OsjDIR genes respond to a wide range of environmental factors, and most OsjDIR genes have a high expression level in roots. Qualitative reverse transcription PCR assays confirmed the responsiveness of OsjDIR genes to the undersupply of mineral elements, the excess of heavy metals and the infection of Rhizoctonia solani. Furthermore, there exist extensive interactions between DIR family members. Taken together, our results shed light on and provide a research foundation for the further exploration of DIR genes in rice.
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DOI：
10.1074/jbc.m114.611780
发表时间：
2015-01-16
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Kim KW;Smith CA;Daily MD;Cort JR;Davin LB;Lewis NG
通讯作者：
Lewis NG
影响因子：
5.1
作者：
Corbin, Cyrielle;Drouet, Samantha;Hano, Christophe
通讯作者：
Hano, Christophe
影响因子：
5.1
作者：
Dalisay, Doralyn S.;Kim, Kye Won;Lewis, Norman G.
通讯作者：
Lewis, Norman G.
影响因子：
10.7
作者：
Kumar, Sudhir;Stecher, Glen;Tamura, Koichiro
通讯作者：
Tamura, Koichiro
影响因子：
13.8
作者：
Cao W;Zhang H;Zhou Y;Zhao J;Lu S;Wang X;Chen X;Yuan L;Guan H;Wang G;Shen W;De Vleesschauwer D;Li Z;Shi X;Gu J;Guo M;Feng Z;Chen Z;Zhang Y;Pan X;Liu W;Liang G;Yan C;Hu K;Liu Q;Zuo S
通讯作者：
Zuo S