The effects of a liquid ethanol diet on nutritional status and fluid balance in the rat.
The effects of a liquid ethanol diet on nutritional status and fluid balance in the rat.
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DOI：
10.1093/alcalc/36.4.298
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发表时间：
2001-07
影响因子：
2.8
通讯作者：
M. Piano;J. Artwohl;S. D. Kim;G. Gass
中科院分区：
文献类型：
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作者：
M. Piano;J. Artwohl;S. D. Kim;G. Gass
液体乙醇饮食是一种广泛使用的乙醇给药方法。本研究的目的是在引入液体乙醇饲料期间和之后，使用多种生理参数（电解质、渗透压摩尔浓度、总血清蛋白、液体摄入/排出和体重）评价液体平衡。将动物随机分为四个不同的饮食方案（两个对照组和两个乙醇组），并将其置于代谢笼中16天。在引入含9%（v/v）乙醇饲料（Lieber-DeCarli：LD）之前、期间和之后1周测量血清电解质以及上述参数。在9%（v/v）乙醇LD的第一个晚上后，动物的饮食消耗量，尿量和体重显着下降。然而，本研究的一个主要发现是，在习惯阶段，电解质值保持在大鼠的正常范围内，特别是在本研究中测量的任何时间点血清钠均未改变。根据本研究的结果，建议在开始液体乙醇饲料期间仔细监测体重，作为动物平衡和生理适应的指标，因为血清钠和计算的渗透压摩尔浓度值均未发生变化。我们的研究结果还强调了在乙醇消费的习惯阶段向动物提供水的必要性。这是因为，尽管摄入了相同量的LD饮食，但自由饮水的乙醇大鼠比不自由饮水的大鼠体重减轻得更少。
The liquid ethanol diet is a widely used method of ethanol administration. The purpose of this study was to evaluate fluid balance using a multitude of physiological parameters (electrolytes, osmolality, total serum proteins, fluid intake/output and body weight), during and after the introduction of liquid ethanol diet. Animals were randomized into four different dietary protocols (two control and two ethanol groups) and were placed in metabolic cages for 16 days. Serum electrolytes, as well as the above parameters, were measured before, during and 1 week after the introduction of 9% (v/v) ethanol-containing diet (Lieber-DeCarli: LD). After the first night on 9% (v/v) ethanol LD, animals had significantly decreased diet consumption, urine output and body weight. However, a major finding of this study was that, during the habituation phase, the electrolyte values remained within the normal range for rats and, in particular, serum sodium was not altered at any time point measured in this study. Based upon the findings from this study, it is recommended that body weight be carefully monitored as a measure of the animal's equilibration and physiological adaptation during the initiation of a liquid ethanol diet, since neither the serum sodium nor calculated osmolality values were changed. Our results also highlight the need to offer water to animals during the habituation phase of ethanol consumption. This is because ethanol rats that were offered water ad libitum lost less weight than groups that did not receive water ad libitum, despite consuming the same amount of LD diet.