The effects of substrate-adsorbed albumin on platelet spreading.
The effects of substrate-adsorbed albumin on platelet spreading.
复制标题
DOI：
10.1163/156856291x00133
复制
发表时间：
1991-01-01
期刊：
影响因子：
--
通讯作者：
Albrecht, R M
中科院分区：
文献类型：
--
作者：
Goodman, S L;Cooper, S L;Albrecht, R M
吸附的白蛋白似乎可以钝化几乎所有材料，最大限度地减少血小板粘附和血栓形成。由于体外血小板扩散可以作为导致血栓形成的体内反应性的指标，并且体外血小板粘附研究通常在牛或人血清白蛋白（BSA或HSA）存在的情况下进行，因此我们研究了白蛋白对血小板对材料底物反应性的影响。在存在足以形成吸附的单层或多层的大量白蛋白浓度的情况下，检查血小板在粘附到几种相关聚氨酯和Formvar上之后的铺展。不存在其他外源蛋白质。使用广义线性交互模型（GLIM）分析粘附血小板的扩散行为。模型表明，无论白蛋白浓度如何，聚合物类型总是影响血小板反应。在许多实验中，可以充分模拟血小板行为，而无需考虑白蛋白的影响。因此，聚合物类型似乎是血小板形状变化的主要决定因素，而吸附的白蛋白产生次要影响。此外，观察到 HSA 和 BSA 对扩散的影响略有不同，这表明人血小板和 HSA 之间的相互作用与常用于血小板制剂中的 BSA 之间存在质的不同。
Adsorbed albumin appears to passivate nearly all materials, minimizing platelet adhesion and thrombus formation. Since in vitro platelet spreading can be an indicator of in vivo reactivity leading to thrombosis, and as in vitro platelet adhesion investigations are routinely done in the presence of bovine or human serum albumin (BSA or HSA), we examined the influence of albumin on platelet reactivity to material substrates. Platelet spreading was examined subsequent to adherence onto several related polyurethanes, and to Formvar, in the presence of bulk albumin concentrations sufficient to form an adsorbed monolayer or a multilayer. No other exogenous proteins were present. The spreading behavior of adherent platelets was analyzed using generalized linear interactive modeling (GLIM). The models showed that the polymer type always influenced platelet responses, irrespective of the albumin concentration. In many experiments, platelet behavior could be adequately modeled without including the effects of albumin. Thus, the polymer type appeared to be the primary determinant of platelet shape-change with adsorbed albumin producing a secondary effect. Additionally, somewhat different effects on spreading were observed with HSA and BSA, suggesting qualitatively different interactions between human platelets and HSA, than with BSA, which is commonly used in platelet preparations.