Selective and sensitive detection of cysteine in water and live cells using a coumarin–Cu2+ fluorescent ensemble
Selective and sensitive detection of cysteine in water and live cells using a coumarin–Cu2+ fluorescent ensemble
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DOI：
10.1039/c8nj03809k
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发表时间：
2018-09
影响因子：
3.3
通讯作者：
Yue Wang;Qingtao Meng;Qian Han;Guangjie He;Yaoyun Hu;Huan Feng;Hongmin Jia;Run Zhang;Zhiqiang Z
中科院分区：
文献类型：
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作者：
Yue Wang;Qingtao Meng;Qian Han;Guangjie He;Yaoyun Hu;Huan Feng;Hongmin Jia;Run Zhang;Zhiqiang Z
由于半胱氨酸在生物系统中的重要作用，半胱氨酸与生物硫醇和其他氨基酸的有效区分受到越来越多的关注。在这项工作中，我们报告了一种新的策略，用于检测Cys在水介质和活细胞中使用一个简单的Cu 2+合奏（L-Cu 2+）。首次合成并表征了香豆素水杨醛腙荧光配体（L）。L对Cu 2+具有高亲和力（Ka = 1.995 × 106 M−1），并在HEPES缓冲液中有效淬灭荧光。原位产生的化学传感系综，L-Cu 2+，能够通过置换方法特异性地响应于Cys而不是其他生物硫醇和氨基酸。结果，可以观察到荧光和紫外-可见吸收光谱的显著恢复。L-Cu ~（2+）对Cys具有较高的选择性和灵敏度。检测限估计为15 nM。以L-Cu ~（2+）为荧光探针，成功地实现了Cys在A549细胞中的成像。
Effective discrimination of cysteine (Cys) from biothiols and other amino acids has gained increasing attention due to the essential roles of this molecule in biological systems. In this work, we report a new strategy for the detection of Cys in aqueous media and live cells using a simple Cu2+ ensemble (L–Cu2+). A coumarin–salicylaldehyde hydrazone fluorescent ligand (L) was initially synthesized and well characterized. L features high affinity towards Cu2+ (Ka = 1.995 × 106 M−1), accompanied by effective quenching of fluorescence in HEPES buffer. The in situ generated chemosensing ensemble, L–Cu2+, is able to specifically respond to Cys over other biothiols and amino acids through a displacement approach. As a result, a remarkable recovery of fluorescence and UV-vis absorption spectra can be observed. L–Cu2+ showed high selectivity and sensitivity towards Cys. The detection limit is estimated to be 15 nM. Using L–Cu2+ as the fluorescent probe, imaging of Cys in live A549 cells was successfully demonstrated.