RAPID LETHAL EFFECT IN RATS OF A THIRD COMPONENT FOUND UPON FRACTIONATING TOXIN OF BACILLUS ANTHRACIS
RAPID LETHAL EFFECT IN RATS OF A THIRD COMPONENT FOUND UPON FRACTIONATING TOXIN OF BACILLUS ANTHRACIS
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DOI：
10.1128/jb.83.6.1274-1280.1962
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发表时间：
1962-01-01
影响因子：
3.2
通讯作者：
THORNE, CB
中科院分区：
文献类型：
--
作者：
BEALL, FA;TAYLOR, MJ;THORNE, CB
研究发现，与几种被认为对炭疽病抵抗力较低的物种相比，老鼠在体外更容易受到炭疽杆菌产生的毒素的致命影响。与老鼠相比，每克体重的毒素杀死老鼠的速度要快得多。豚鼠在致死老鼠的毒素剂量下幸存下来。静脉注射Fischer 344大鼠是一种快速的致死活性测试，它有助于证明毒素中与保护性抗原不同的两种成分。其中一种是致命因子，从另一种导致豚鼠皮肤浮肿的成分中分离出来。后一种成分不是致死作用所必需的。除非与保护性抗原结合，否则这两种因子都不起作用。虽然豚鼠皮肤反应已被常规用来检测样品的毒性，但目前的结果表明，这项测试不能检测致死成分。
Rats were found to be more susceptible to the lethal effect of toxin produced by Bacillus anthracis in vitro than were several species considered less resistant to anthrax. Rats were killed much faster by less toxin per gram of body weight than were mice. Guinea pigs survived doses of toxin that killed rats. Intravenous injection of Fischer 344 rats is a rapid test for lethal activity, which facilitates the demonstration of two components, different from protective antigen, in toxin. One of these, a lethal factor, was separated from the other component, which causes cutaneous edema in the guinea pig. The latter component was not necessary for lethal effect. Neither of these factors was active unless combined with protective antigen. Although the guinea pig skin reaction has been used routinely to assay the toxicity of samples, the present results show that this test does not assay the lethal component.