Bladder Telemetry: A New Approach to Evaluate Micturition Behavior Under Physiological and Inflammatory Conditions
Bladder Telemetry: A New Approach to Evaluate Micturition Behavior Under Physiological and Inflammatory Conditions
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DOI：
10.1002/nau.22970
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发表时间：
2017-02-01
影响因子：
2
通讯作者：
Junquero, Didier
中科院分区：
文献类型：
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作者：
Monjotin, Nicolas;Farrie, Martine;Junquero, Didier
目的：建立一种利用遥测技术对有意识大鼠进行膀胱造瘘的新方法，并利用该技术确定在生理、炎症和疼痛条件下，有意识决策过程在开始排尿方面的作用。方法：将遥测式压力传感器植入膀胱穹窿内。在至少1个月的恢复期后，用速尿脉冲激活利尿后进行了一些尿动力学参数的调查。生理条件下用托特罗定和米拉贝隆对模型进行表征，并重复使用相同的动物，观察环磷酰胺诱导膀胱炎症对排尿模式的影响。结果：遥测记录的痕迹质量和参数测量与常规膀胱造口术相当。速尿引起可重复的短暂尿量增加和一系列持续60min的尿空。托特罗定减少了排尿收缩的幅度，而米拉贝龙没有任何影响。注射CYP后7h，排尿频率明显增加，排尿幅度收缩无明显变化。然而，每次排尿时的平均排尿量和总排尿量减少。注射CYP 24H后第二次暴露速尿，排尿模式恢复到对照组，但排尿量仍低于对照组。结论：这种遥测模型似乎与以前在有意识的传统膀胱测量中描述的一样准确，并且允许重复评估可能调节排尿模式的化合物。(C) 2016年作者。Wiley期刊公司出版的神经病学和泌尿动力学。
Aims: To establish a new approach to cystometry using telemetry in conscious rats and to use this technique to determine the role of conscious decision making processes with respect to the initiation of voiding in physiological, inflammatory, and painful conditions. Methods: The pressure transducer of a telemetric transmitter was implanted in the dome of the urinary bladder. After a recovery period of at least 1 month, several investigations of urodynamic parameters were performed after diuresis activation by a pulse of furosemide. The model was characterized by tolterodine and mirabegron under physiological conditions and same animals were reused to evaluate the modification of the voiding pattern under bladder inflammation induced by cyclophosphamide. Results: The quality of traces and measurement of parameters recorded telemetrically were comparable to those with conventional cystometry. Furosemide induced a reproducible transient increase of urine production and a series of voids that persisted for 60min. Tolterodine reduced the amplitude of micturition contractions although mirabegron was devoid of any effect. Seven hours after injection of CYP, voiding frequency increased significantly and the micturition amplitude contraction was not altered. However, the mean volume voided during individual micturitions and the total voided volume decreased. During a second exposure to furosemide 24H after CYP injection, the micturition pattern returned to control, however, the micturition volume was still lower than in control. Conclusion: This telemetric model appears to be as accurate as previously described in conscious conventional cystometry, and allows the repeated evaluation of compounds which may modulate the voiding patterns. (C) 2016 The Authors. Neurourology and Urodynamics published by Wiley Periodicals, Inc.