Ultraviolet-B radiation effects on phenolic profile and flavonoid content of Kalanchoe pinnata
Ultraviolet-B radiation effects on phenolic profile and flavonoid content of Kalanchoe pinnata
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DOI：
10.1016/j.jphotobiol.2015.03.011
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发表时间：
2015-07-01
影响因子：
5.4
通讯作者：
Tavares, Eliana Schwartz
中科院分区：
文献类型：
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作者：
dos Santos Nascimento, Luana Beatriz;Leal-Costa, Marcos Vinicius;Tavares, Eliana Schwartz
紫外线-B辐射是一种重要的非生物因子，可以刺激次级代谢产物的产生，包括多酚化合物。羽叶吊兰（景天科）是一种药用植物，在巴西广泛用于治疗伤口和炎症。该物种富含酚类化合物，这可以解释其一些生物活性，包括抗利什曼病，抗高血压和抗菌特性。研究了UV-B辐射对K.状脉植物在白色光（W -对照）和补充UV-B辐射（W + UVB）下生长。补充UV-B辐射提高了叶提取物的总黄酮含量，而不影响提取物的抗氧化活性或产量。W和W + UVB叶提取物的TLC和HPLC分析揭示了其酚类化合物的定量和定性差异。W + UVB提取物含有较高的多样性的酚类化合物和大量的槲皮苷，一个重要的生物活性黄酮的这种物种。这是使用ImageJ（R）程序分析通过喷雾NP-PEG试剂可视化的TLC的第一份报告。建议将UV-B辐射作为K.羽叶栽培，以提高其黄酮类成分。(C)2015爱思唯尔B. V.保留所有权利。
Ultraviolet-B radiation is an important abiotic factor that can stimulate the production of secondary metabolites, including polyphenolic compounds. Kalanchoe pinnata (Crassulaceae) is a medicinal plant popularly used in Brazil for treating wounds and inflammation. This species is rich in phenolic compounds, which could account for some of its biological activities, including antileishmanial, antihypertensive and antibacterial properties. We investigated the effects of supplemental UV-B radiation on the phenolic profile, antioxidant activity and total flavonoid content of leaves of K. pinnate. Plants were grown under white light (W - control) and supplemental UV-B radiation (W + UVB). Supplemental UV-B radiation enhanced the total flavonoid content of the leaf extracts, without affecting the antioxidant activity or yield of extracts. Analysis by TLC and HPLC of W and W + UVB leaf extracts revealed quantitative and qualitative differences in their phenolic profiles. W + UVB extracts contained a higher diversity of phenolic compounds and a larger amount of quercitrin, an important bioactive flavonoid of this species. This is the first report of the use of ImageJ (R) program to analyze a TLC visualized by spraying with NP-PEG reagent. UV-B radiation is proposed as a supplemental light source in K. pinnata cultivation in order to improve its flavonoid composition. (C) 2015 Elsevier B.V. All rights reserved.