GRAPEVINE STILBENE SYNTHASE CDNA ONLY SLIGHTLY DIFFERING FROM CHALCONE SYNTHASE CDNA IS EXPRESSED IN ESCHERICHIA-COLI INTO A CATALYTICALLY ACTIVE ENZYME
GRAPEVINE STILBENE SYNTHASE CDNA ONLY SLIGHTLY DIFFERING FROM CHALCONE SYNTHASE CDNA IS EXPRESSED IN ESCHERICHIA-COLI INTO A CATALYTICALLY ACTIVE ENZYME
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DOI：
10.1016/0014-5793(90)80961-h
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发表时间：
1990-07-30
期刊：
影响因子：
3.5
通讯作者：
KINDL, H
中科院分区：
文献类型：
--
作者：
MELCHIOR, F;KINDL, H
芪合酶负责白藜芦醇和其他芪类化合物的形成，这些化合物在葡萄藤中作为植物抗毒素发挥作用。从葡萄藤mRNA中制备了一个芪合酶的全长cDNA，并进行了测序。将pSV 25中编码392个氨基酸多肽的插入片段分别插入载体pKK 233 -2和pDS 12/RBSII-2中。在大肠杆菌中表达的cDNA产生的酶活性二聚体表现出单独的芪合酶活性。通过酶活性和Western blot分析对该蛋白进行了表征。
Stilbene synthase is responsible for the formation of resveratrol and other stilbenes which function in grapevine as phytoalexins. A full-length stilbene synthase cDNA was prepared from grapevine mRNA and sequenced. The insert in pSV25 coding for a polypeptide with 392 amino acids was inserted into the vectors pKK233-2 and pDS12/RBSII-2, respectively. Expression of the cDNA inEscherichia coliyielded an enzyniatically active dimer exhibiting solely stilbene synthase activity. The protein was characterized by enzyme activity and Western blot analysis.