Array-based sensing of proteins using conjugated polymers
Array-based sensing of proteins using conjugated polymers
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DOI：
10.1021/ja0737927
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发表时间：
2007-08-15
影响因子：
15
通讯作者：
Rotello, Vincent M.
中科院分区：
文献类型：
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作者：
Miranda, Oscar R.;You, Chang-Cheng;Rotello, Vincent M.
构建了一个由六种功能化聚（对苯乙烯）聚合物组成的传感器阵列，用于检测和鉴定蛋白质分析物。蛋白质的存在改变了聚合物的荧光特性，对给定的蛋白质产生不同的荧光响应模式。线性判别分析准确区分17种蛋白质在纳米至微摩尔浓度的模式。在对来自训练集的68个未知蛋白质样本进行测试的基础上，鉴定准确率达到97%。
A sensor array comprising six functionalized poly(p-phenyleneethynylene) polymers was constructed and used to detect and identify protein analytes. The presence of proteins alters the fluorescence properties of the polymers, generating a distinct fluorescence response pattern for a given protein. Linear discriminant analyses accurately differentiate the patterns of 17 proteins at nano- to micromolar concentrations. An identification accuracy of 97% was obtained on the basis of the tests with 68 unknown protein samples from the training set.