Multiple Functions for Drosophila Mcm10 Suggested Through Analysis of Two Mcm10 Mutant Alleles
Multiple Functions for Drosophila Mcm10 Suggested Through Analysis of Two Mcm10 Mutant Alleles
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DOI：
10.1534/genetics.110.117234
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发表时间：
2010-08-01
期刊：
影响因子：
3.3
通讯作者：
Christensen, Tim W.
中科院分区：
文献类型：
--
作者：
Apger, Jennifer;Reubens, Michael;Christensen, Tim W.
DNA复制和DNA正确包装成不同状态的染色质在所有真核细胞中都是必不可少的过程。DNA的高保真复制对于遗传物质向细胞的传播至关重要。同样，保持表观遗传的染色质状态对于细胞转录状态的忠实复制是必不可少的。越来越明显的是，这两个过程通过DNA复制蛋白和染色质相关蛋白之间的相互作用而联系在一起。此外，越来越多的蛋白质正在被发现，它们在DNA复制和维持表观遗传状态方面都具有双重作用。我们对两个果蝇突变的保守DNA复制蛋白Mcm10进行了分析。一个亚型突变表明Mcm10在异色沉默和染色体凝聚中起作用，而对Mcm10一个新的C端截断等位基因的分析表明，与Mcm2的相互作用不是染色体凝聚和异色沉默所必需的，但对DNA复制是重要的。
DNA replication and the correct packaging of DNA into different states of chromatin are both essential processes in all eukaryotic cells. High-fidelity replication of DNA is essential for the transmission of genetic material to cells. Likewise the maintenance of the epigenetic chromatin states is essential to the faithful reproduction of the transcriptional state of the cell. It is becoming more apparent that these two processes are linked through interactions between DNA replication proteins and chromatin-associated proteins. In addition, more proteins are being discovered that have dual roles in both DNA replication and the maintenance of epigenetic states. We present an analysis of two Drosophila mutants in the conserved DNA replication protein Mcm10. A hypomorphic mutant demonstrates that Mcm10 has a role in heterochromatic silencing and chromosome condensation, while the analysis of a novel C-terminal truncation allele of Mcm10 suggests that an interaction with Mcm2 is not required for chromosome condensation and heterochromatic silencing but is important for DNA replication.