Study of antimutagenic and antioxidant activities of Gallic acid and 1,2,3,4,6-pentagalloylglucose from Pistacia lentiscus -: Confirmation by microarray expression profiling
Study of antimutagenic and antioxidant activities of Gallic acid and 1,2,3,4,6-pentagalloylglucose from Pistacia lentiscus -: Confirmation by microarray expression profiling
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DOI：
10.1016/j.cbi.2006.10.003
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发表时间：
2007-01-05
影响因子：
5.1
通讯作者：
Chekir-Ghedira, Leila
中科院分区：
文献类型：
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作者：
Abdelwahed, Afef;Bouhlel, Ines;Chekir-Ghedira, Leila
研究了从黄连木果实中分离的两种多酚的体外抗氧化和抗诱变活性。通过对自由基1,1-二苯基-2-picrylhydrazyl （DPPH中心点）的清除能力、对黄嘌呤氧化酶的抑制能力和对H2O2诱导的K562细胞脂质过氧化的抑制能力来测定各化合物的抗氧化活性。以大肠杆菌PQ37为测试菌株，采用SOS染色法测定抗诱变活性，以K562细胞株采用Comet法测定抗诱变活性。1,2,3,4,6-五五烯基葡萄糖具有清除DPPH中心点自由基和防止脂质过氧化的作用。此外，这两种化合物对硝呋虫胺和黄曲霉毒素b1诱变具有抑制活性。这些分子所表现出的保护作用也通过对氧化应激反应的基因表达分析来确定。为此，我们使用了包含82个与细胞防御相关的基因的DNA微阵列，主要由抗氧化和DNA修复蛋白代表。我们发现1,2,3,4,6-五戊烯基葡萄糖诱导了11个与抗氧化酶家族相关的转录本（GPX1， TXN, AOE372， SHC1和SEPW1）和DNA修复（POLD1， APEX, POLD2， MPG， PARP和XRCC5）的表达降低。使用没食子酸，诱导TXN、TXNRDI、AOE372、GSS（抗氧化酶）和LIG4、POLD2、MPG、GADD45A、PCNA、RPA2、DDIT3、HMOX2、XPA、TDG、ERCC1和GTF2H1 （DNA修复）基因的表达，抑制GPX1、SEPWI、POLD1和SHC1基因的表达。2006爱思唯尔爱尔兰有限公司版权所有。
In vitro antioxidant and antimutagenic activities of two polyphenols isolated from the fruits of Pistacia lentiscus was assessed. Antioxidant activity was determined by the ability of each compound to scavenge the free radical 1,1-diphenyl-2-picrylhydrazyl (DPPH center dot), to inhibit xanthine oxidase and to inhibit the lipid peroxidation induced by H2O2 in K562 cell line. Antimutagenic activity was assayed with SOS ehromotest using Escherichia coli PQ37 as tester strain and Comet assay using K562 cell line. 1,2,3,4,6-Pentagalloylglucose was found to be more effective to scavenge DPPH center dot radical and protect against lipid peroxidation. Moreover, these two compounds induced an inhibitory activity against nifuroxazide and aflatoxin B 1 mutagenicity. The protective effect exhibited by these molecules was also determined by analysis of gene expression as response to an oxidative stress. For this purpose, we used a cDNA-microarray containing 82 genes related to cell defense, essentially represented by antioxidant and DNA repair proteins. We found that 1,2,3,4,6-pentagalloylglucose induced a decrease in the expression of 11 transcripts related to antioxidant enzymes family (GPX1, TXN, AOE372, SHC1 and SEPW1) and DNA repair (POLD1, APEX, POLD2, MPG, PARP and XRCC5). The use of Gallic acid, induced expression of TXN, TXNRDI, AOE372, GSS (antioxidant enzymes) and LIG4, POLD2, MPG, GADD45A, PCNA, RPA2, DDIT3, HMOX2, XPA, TDG, ERCC1 and GTF2H1 (DNA repair) as well as the repression of GPX1, SEPWI, POLD1 and SHC1 gene expression. (c) 2006 Elsevier Ireland Ltd. All rights reserved.