Evaluation of the analytical performances of a portable, 18-parameter hemometric system using capillary blood samples for blood donor enrolment
Evaluation of the analytical performances of a portable, 18-parameter hemometric system using capillary blood samples for blood donor enrolment
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DOI：
10.1111/j.1423-0410.2009.01256.x
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发表时间：
2010-02-01
期刊：
影响因子：
2.7
通讯作者：
Mannella, E.
中科院分区：
文献类型：
--
作者：
Pierelli, L.;Zennaro, F.;Mannella, E.
背景和目的献血者的登记过程通常基于单个毛细血管血红蛋白(Hb)的评估，而采集法的血小板捐献者还需要进行血小板(Plt)计数。“唯一的HB”方法妨碍了对捐赠者的完整评估，并且不允许Plt捐赠者登记。为了延长献血前的血细胞计数，我们评估了使用毛细血管血液的多参数计数器的性能。材料和方法ABX Micros 60(Micros 60)血液分析仪用于毛细血管血，并与参考计数器(Coulter Act 5diff)在第一个系列的416名献血者和第二个系列的136名献血者在常规使用3个月后的静脉计数进行比较。平均采集L毛细血管血50亩，其中L毛细血管血10亩。结果Micros 60毛细血管计数与参比计数器静脉计数高度相关。在第一系列比较中，毛细管法的平均血小板计数相差37×109/L，但在第二系列比较中，由于操作员在毛细管采样方面有更多的专业知识，血小板计数减少到10×109/L。所有其他参数都是准确的，从未达到临床意义，尽管它们显示出统计学上的显著差异。结论Micros60上的数据表明，毛细血管预献血计数可能是实现献血和Plt捐献者准确登记过程的一种可行和有效的方法。
Background and ObjectivesBlood donor enrolment process is frequently based on the sole capillary haemoglobin (Hb) evaluation while platelet donors by apheresis also requires platelet (Plt) count. The 'sole Hb' approach prevents a complete donor evaluation and does not allow Plt donor enrolment. To extend blood counts before donations, we evaluated the performances of a multiparametric counter using capillary blood.Materials and MethodsThe ABX Micros 60 (Micros 60) blood analyzer was employed on capillary blood and compared with venous counts by a reference counter (Coulter AcT 5diff) in a first series of 416 donors and in a second series of 136, after a 3-month period of routine use of this study counter. An average of 50 mu l of capillary blood was collected whose 10 mu l had been aspirated by Micros 60.ResultsHigh correlations were found between capillary counts using Micros 60 and venous counts using the reference counter. Mean Plt counts differed of 37 x 109/l less for capillary approach in the first series of comparisons, but decreased to 10 x 109/l less in the second series due to a greater expertise of operators in capillary sampling. All other parameters were accurate and never reached clinical relevance albeit they showed statistically significant differences.ConclusionData on Micros 60 demonstrated that capillary predonation counts may represent a feasible and effective approach to realize an accurate enrolment process of blood and Plt donors.