Centromeric localization of dispersed Pol III genes in fission yeast.
Centromeric localization of dispersed Pol III genes in fission yeast.
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DOI：
10.1091/mbc.e09-09-0790
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发表时间：
2010-01-15
影响因子：
3.3
通讯作者：
Noma K
中科院分区：
文献类型：
--
作者：
Iwasaki O;Tanaka A;Tanizawa H;Grewal SI;Noma K
作者表明，Pol III转录的基因，如tRNA和5S rRNA基因定位于裂殖酵母的着丝粒。Pol III基因的着丝粒定位由凝聚素介导。这项研究表明，分散的Pol III基因的着丝粒定位和有丝分裂染色体凝聚之间存在功能联系。真核生物基因组是一个复杂的三维实体，位于细胞核中。我们提出的证据表明，Pol III转录的基因，如tRNA和5S rRNA基因可以定位于着丝粒，并有助于全球基因组组织。此外，我们发现，异位插入Pol III基因到一个非Pol III基因位点的结果在着丝粒定位的轨迹。我们发现，着丝粒定位的Pol III基因介导的凝聚素，它与Pol III的转录机制相互作用，和转录水平的Pol III基因的Pol III基因的着丝粒定位呈负相关。这种着丝粒定位的Pol III基因最初观察到在间期变得突出有丝分裂过程中，当染色体浓缩。值得注意的是，有缺陷的有丝分裂染色体凝聚的凝聚素突变，cut 3 -477，这减少了着丝粒定位的Pol III基因，被抑制的突变，在sfc 3基因编码的Pol III转录因子TFIIIC亚基，sfc 3 -1。sfc 3 -1突变促进Pol III基因的着丝粒定位。我们的研究表明，分散的Pol III基因的着丝粒定位过程，它们的转录和凝聚的有丝分裂染色体的组装之间存在功能联系。
The authors show that Pol III transcribed genes such as tRNA and 5S rRNA genes localize to centromeres in fission yeast. The centromeric localization of Pol III genes is mediated by condensin. This study suggests that there is a functional link between the centromeric localization of dispersed Pol III genes and mitotic chromosome condensation. The eukaryotic genome is a complex three-dimensional entity residing in the nucleus. We present evidence that Pol III–transcribed genes such as tRNA and 5S rRNA genes can localize to centromeres and contribute to a global genome organization. Furthermore, we find that ectopic insertion of Pol III genes into a non-Pol III gene locus results in the centromeric localization of the locus. We show that the centromeric localization of Pol III genes is mediated by condensin, which interacts with the Pol III transcription machinery, and that transcription levels of the Pol III genes are negatively correlated with the centromeric localization of Pol III genes. This centromeric localization of Pol III genes initially observed in interphase becomes prominent during mitosis, when chromosomes are condensed. Remarkably, defective mitotic chromosome condensation by a condensin mutation, cut3-477, which reduces the centromeric localization of Pol III genes, is suppressed by a mutation in the sfc3 gene encoding the Pol III transcription factor TFIIIC subunit, sfc3-1. The sfc3-1 mutation promotes the centromeric localization of Pol III genes. Our study suggests there are functional links between the process of the centromeric localization of dispersed Pol III genes, their transcription, and the assembly of condensed mitotic chromosomes.
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影响因子：
7.8
作者：
Lawrence, Jeanne B.;Clemson, Christine M.
通讯作者：
Clemson, Christine M.
影响因子：
10.5
作者：
Dubey, Rudra N.;Gartenberg, Marc R.
通讯作者：
Gartenberg, Marc R.
影响因子：
10.5
作者：
D'Ambrosio, Claudio;Schmidt, Christine Katrin;Uhlmann, Frank
通讯作者：
Uhlmann, Frank
DOI：
10.1073/pnas.232688099
发表时间：
2003-01-07
影响因子：
11.1
作者：
Hall, IM;Noma, K;Grewal, SIS
通讯作者：
Grewal, SIS
影响因子：
7.5
作者：
Belmont, Andrew S.
通讯作者：
Belmont, Andrew S.