Antifungal Activities of Metabolites Produced by a Termite-Associated Streptomyces canus BYB02
Antifungal Activities of Metabolites Produced by a Termite-Associated Streptomyces canus BYB02
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白蚁相关链霉菌 BYB02 产生的代谢物的抗真菌活性
DOI：
10.1021/jf305210u
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发表时间：
2013-02-20
影响因子：
6.1
通讯作者：
Xu, Jia-dong
中科院分区：
文献类型：
--
作者：
Zhang, Ying-lao;Li, Shuai;Xu, Jia-dong
采用柱层析法从白蚁相关的canus链霉菌BYBO2中分离纯化了两种主要的抗真菌代谢产物resistance omycin和tetracenomycin D。在广泛的光谱分析的基础上确定了分离化合物的结构。耐药霉素对蘑菇病菌（Valsa mali）和稻瘟病病菌（Magnaporthe grisea）的IC50值分别为1.1 μ g/mL和3.8 μ g/mL， IC50值与参比环己内酯相当，分别为2.3和0.3 pg/mL。进一步的孢子萌发试验表明，耐药霉素对稻瘟病菌的孢子萌发有明显的抑制作用，IC50值为5.55 μ g/mL。最后，体内抗真菌活性实验表明，耐药霉素对稻瘟病菌具有显著的预防效果，其防治效果分别为66.8和58.7%，高于对照多菌灵。目前的研究结果表明，耐药霉素具有作为杀菌剂的潜力。
Two main antifimgal metabolites resistomycin and tetracenomycin D were isolated and purified from a termiteassociated Streptomyces canus BYBO2 by column chromatography. The structures of isolated compounds were determined on the basis of extensive spectroscopic analysis. Resistomycin possessed strong activities against mycelial growth of Valsa mali (IC50 = 1.1 mu g/mL) and Magnaporthe grisea (IC50 = 3.8 mu g/mL), which were comparable to those of referenced cyclohexirnide, with IC50 values of 2.3 and 0.3 pg/mL, respectively. A further spore germination test showed that resistomycin exhibited potent reduction in spore germination for M. grisea, with an IC50 value of 5.55 mu g/mL. Finally, the in vivo antifungal activity experiment showed that resistomycin possessed significant preventive efficacy against rice blast, which was more potent than that of referenced carbendazim, with control efficacies of 66.8 and 58.7%, respectively. The present results suggest that resistomycin has potential to be used as a fungicide.