MYB19LONG is involved in brushmark pattern development in Asiatic hybrid lily (Lilium spp.) flowers
MYB19LONG is involved in brushmark pattern development in Asiatic hybrid lily (Lilium spp.) flowers
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MYB19LONG 参与亚洲杂交百合（百合属）花的刷痕图案发育
DOI：
10.1016/j.scienta.2020.109570
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发表时间：
2020
影响因子：
4.3
通讯作者：
Masumi Yamagishi
中科院分区：
文献类型：
--
作者：
Shumpei Goda;Toshiki Hatanaka;Takashi Akagi;Yasutaka Kubo;Ryohei Nakano and Koichiro Ushijima;Masumi Yamagishi;Masumi Yamagishi
亚洲百合品种“Centerfold”和“洛雷托”在花被片的内表面上形成花青苷刷痕颜色图案，其由沿着主维管束产生的一组条纹组成。为了阐明刷痕模式的潜在机制，在两个品种中筛选了正调控花青素合成的R2 R3-MYB转录因子，并鉴定了MYB 19 LONG（MYB 19 L）和MYB 19 SHORT（MYB 19 S）。其中MYB 19 L基因的表达谱与花色素苷合成基因和花被片色素沉积基因的表达谱具有较好的相关性。由于MYB 19和MYB 19 S是调控百合花被片上凸起斑点着色的转录因子，因此利用F1群体分离刷痕和凸起斑点颜色模式，研究MYB 19 L与刷痕和凸起斑点颜色模式的相关性，发现MYB 19 L在产生刷痕的F1植株中始终表达，表明MYB 19 L是刷痕着色所必需的。F1代只产生凸起斑点的植株表达MYB 19 L、MYB 19 S或两者都表达，表明MYB 19 L也参与凸起斑点的色素沉着。F1代群体的分离结果表明，MYB 19 L是刷痕形成的必要基因，但其他基因也参与了刷痕的形成。最后，讨论了MYB 19 Lin在刷纹和凸点形成中的作用。
Asiatic hybrid lily (Liliumspp.) cultivars ‘Centerfold’ and ‘Loreto’ developed an anthocyanin brushmark color pattern on the interior surfaces of flower tepals, which consisted of a set of streaks generated along the main vascular bundles. To clarify the mechanism underlying the brushmark pattern, R2R3-MYB transcription factors that positively regulated anthocyanin biosynthesis were screened in the two cultivars, and MYB19LONG (MYB19L) and MYB19SHORT (MYB19S) were identified. Among them, the expression profile ofMYB19Lcorrelated well with those of anthocyanin biosynthesis genes and pigment deposition in tepals. AsMYB19LandMYB19Swere recently identified as transcription factors regulating the pigmentation of raised spots on lily tepals, the correlation ofMYB19Lwith brushmark and raised spot color patterns was evaluated using F1populations by segregating the color patterns.MYB19Lwas always expressed in the F1plants that developed brushmarks, indicating thatMYB19Lis necessary for brushmark pigmentation. The F1plants that developed only raised spots expressedMYB19L,MYB19S, or both, suggesting thatMYB19Lis also involved in raised spot pigmentation. Segregation of the F1populations showed thatMYB19Lwas necessary but other gene(s) also participated in brushmark development. Finally, the roles ofMYB19Lin the development of brushmarks and raised spots are discussed.