Effect of amiodarone on L-triiodothyronine stimulation of [3H] thymidine incorporation into GH3 cells
Effect of amiodarone on L-triiodothyronine stimulation of [3H] thymidine incorporation into GH3 cells
复制标题
胺碘酮对 L-三碘甲状腺原氨酸刺激 [3H] 胸苷掺入 GH3 细胞的影响
DOI：
--
复制
发表时间：
1982
影响因子：
5.4
通讯作者：
K. Woeber
中科院分区：
文献类型：
--
作者：
I. Goldfine;B. Maddux;K. Woeber
抗心律失常药物胺碘酮可抑制l -三碘甲状腺原氨酸对[3H]胸腺嘧啶并入GH3大鼠垂体肿瘤细胞的刺激作用。在浓度低至0.5 μM时检测到胺碘酮的抑制作用；在2 μM时，l -三碘甲状腺原氨酸的刺激作用大于50%被抑制。在所有浓度的l -三碘甲状腺原氨酸（50 pM至10 nM）测试中，胺碘酮的作用都存在，表明胺碘酮是一种非竞争性拮抗剂。因此，这些研究提出了一种可能性，即胺碘酮对甲状腺激素代谢的影响可能部分是通过在细胞水平上抑制甲状腺激素的作用来介导的。
The antiarrhythmic agent amiodarone was found to inhibit the stimulatory effects of L-triiodothyronine on [3H ] thymidine incorporation into GH3 rat pituitary tumor cells. This inhibitory effect of amiodarone was detected at concentrations as low as 0.5 μM; at 2 μM greater than 50% of the stimulatory effect of L-triiodothyronine was inhibited. The effect of amiodarone was present at all concentrations of L-triiodothyronine tested (50 pM to 10 nM), suggesting that amiodarone acted as a non-competitive antagonist. These studies raise the possibility, therefore, that the effect of amiodarone on thyroid hormone metabolism may be mediated in part by an inhibition of thyroid hormone action at the cellular level.