RAPD analysis on six populations of the leopard cat in China
RAPD analysis on six populations of the leopard cat in China
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发表时间：
2004
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通讯作者：
Su-ying Bai;Yan-chun Xu;Dongliang Zhou;Xiao Ping Li;Songyan Jing;Yu Jin
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作者：
Su-ying Bai;Yan-chun Xu;Dongliang Zhou;Xiao Ping Li;Songyan Jing;Yu Jin
采用随机扩增多态性DNA（RAPD）方法分析了中国豹猫（Prionailurus bengalensis）6个种群的遗传分化和系统发育关系。从40条随机引物中获得35条有效引物。 35条引物分别扩增代表6个群体的6个混合样本，获得288条DNA条带，其中多态性条带231条，占80.21%。单个引物产生的DNA条带数量为2至16条。平均每个引物产生8.23条DNA带。根据遗传距离，采用算术平均配对配对法（UPGMA）构建了豹猫的分子系统发育树，将豹猫的6个居群划分为4个进化枝：黑龙江山西进化枝、贵州桂桂进化枝、云南进化枝和福建进化枝，对应于P. b.埃普蒂拉拉，P. b.孟加拉人，P.b。 scripta 和 P. b.分别是中华草。研究结果支持了基于形态特征和地理分布的豹猫亚种的传统分类法。
Genetic divergence and phylogenetic relationships among six populations of the leopard cat (Prionailurus bengalensis) in China were analyzed by Random Amplified Polymorphic DNAs (RAPD) methods. 35 effective primers were obtained from 40 random primers. 35 primers amplified 6 mixed samples representing 6 populations respectively and 288 DNA bands were obtained, of which 231 bands are polymorphic, accounting for 80.21%. The numbers of DNA bands produced by single primer range from 2 to 16. On average, each primer produces 8.23 DNA bands. Based on genetic distances, molecular phylogenetic trees of the leopard cat have been constructed by Unweighted Pair Group Method using Arithmetic Mean(UPGMA), 6 populations of the leopard cat can be divided into 4 clades: the Heilongjiang-Shanxi clade, the Guizhou-Guangxi clade, the Yunnan clade and the Fujian clade, which correspond to P. b. euptilura, P. b. bengalensis, P. b. scripta and P. b. chinensis, respectively. The results support the traditional taxonomy of leopard cat subspecies based on morphological characteristics and geographic distribution.