Synaptonemal complex assembly in C-elegans is dispensable for loading strand-exchange proteins but critical for proper completion of recombination
Synaptonemal complex assembly in C-elegans is dispensable for loading strand-exchange proteins but critical for proper completion of recombination
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DOI：
10.1016/s1534-5807(03)00232-6
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发表时间：
2003-09-01
影响因子：
11.8
通讯作者：
Villeneuve, AM
中科院分区：
文献类型：
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作者：
Colaiácovo, MP;MacQueen, AJ;Villeneuve, AM
本研究探讨了秀丽隐杆线虫减数分裂过程中突触复合体（SC）的组装与同源染色体间重组进展之间的关系。我们确定SYP-2是SC中心区域的一个结构成分，并表明中心区域的组装依赖于染色体轴的适当形态发生。我们发现SC中心区域对于重组的起始和DNA链交换蛋白RAD-51的装载是必不可少的，尽管事实上广泛的RAD-51装载通常发生在组装SC的背景下。在减数分裂突变体中，RAD-51灶的持续存在和交叉产物的缺失表明，需要SC中心区成分和重组蛋白MSH-4和MSH-5来促进被切除的双链断裂转化为稳定的后链交换中间体。我们的数据还表明，利用姐妹染色单体作为修复模板的早期前期障碍并不取决于中心区域组装。
Here we probe the relationships between assembly of the synaptonemal complex (SC) and progression of recombination between homologous chromosomes during Caenorhabditis elegans meiosis. We identify SYP-2 as a structural component of the SC central region and show that central region assembly depends on proper morphogenesis of chromosome axes. We find that the SC central region is dispensable for initiation of recombination and for loading of DNA strand-exchange protein RAD-51, despite the fact that extensive RAD-51 loading normally occurs in the context of assembled SC. Further, persistence of RAD-51 foci and absence of crossover products in meiotic mutants suggests that SC central region components and recombination proteins MSH-4 and MSH-5 are required to promote conversion of resected double-strand breaks into stable post-strand exchange intermediates. Our data also suggest that early prophase barriers to utilization of sister chromatids as repair templates do not depend on central region assembly.