Association between FOXP3+ regulatory T-cells and occurrence of peritoneal lesions in women with ovarian endometrioma and dermoid cysts
Association between FOXP3+ regulatory T-cells and occurrence of peritoneal lesions in women with ovarian endometrioma and dermoid cysts
复制标题
DOI：
10.1016/j.rbmo.2019.01.011
复制
发表时间：
2019-06-01
影响因子：
4
通讯作者：
Kitawaki, Jo
中科院分区：
文献类型：
--
作者：
Khan, Khaleque N.;Yamamoto, Kazuo;Kitawaki, Jo
研究问题：FOXP3（+）调节性t细胞（Treg）的数量与卵巢子宫内膜异位瘤、皮样囊肿患者腹膜病变的发生是否有关系？设计：回顾性和前瞻性病例对照队列研究。我们收集了27例卵巢子宫内膜异位瘤患者和25例皮样囊肿患者的腹膜病变。本文收集了36例卵巢子宫内膜异位瘤患者和42例皮样囊肿患者的腹膜液。免疫组织化学检测Treg细胞转录因子之一叉头盒P3 （FOXP3）和转化生长因子- β (tgf - β)的组织表达。采用酶联免疫吸附法测定腹膜液中白细胞介素-6 （IL-6）和tgf - β水平。结果：卵巢子宫内膜瘤合并腹膜病变的发生率明显高于合并腹膜病变的皮样囊肿（269/350[76.9%]∶74/414 [17.9%];P < 0.001）。伴有子宫内膜异位瘤（F = 21.52, P < 0.001）和皮样囊肿（F = 22.01, P < 0.001）的女性腹膜病变中FOXP3(+) Treg细胞数量明显高于无腹膜病变的女性。病理病变中较高的FOXP3(+) Treg细胞数量与腹膜病变女性腹膜液中tgf - β (P < 0.001)和IL-6 （P = 0.020）水平显著高于无病变女性。结论：本研究证实了目前的推测，子宫内膜异位症与Treg细胞的改变有关，导致子宫内膜异位症和皮样囊肿患者异位子宫内膜病变的存活和着床。这一发现可能解释了为什么在普通人群中只有10%的女性会发生子宫内膜异位症，尽管在90%的女性中有周期性的逆行血流。
Research question: Is there any relationship between numbers of FOXP3(+) regulatory T-cells (Treg) and occurrence of peritoneal lesions in women with ovarian endometrioma and dermoid cysts?Design: Retrospective and prospective case-controlled cohort study. Peritoneal lesions were collected from 27 women with ovarian endometrioma and 25 women with dermoid cysts. Peritoneal fluid was collected from 36 women with ovarian endometrioma and 42 women with dermoid cysts. Tissue expression of Forkhead box P3 (FOXP3), one of the transcription factors of Treg cells, and transforming growth factor-beta (TGF-beta) were examined by immunohistochemistry. Interleukin-6 (IL-6) and TGF-beta levels in the peritoneal fluid were measured by enzyme-linked immunosorbent assay.Results: Ovarian endometrioma cases with coexisting peritoneal lesions were significantly more frequent than dermoid cyst cases with coexistent peritoneal lesions (269/350 [76.9%] versus 74/414 [17.9%]; P < 0.001). Numbers of FOXP3(+) Treg cells were significantly higher in peritoneal lesions of women coexistent with ovarian endometrioma (F = 21.52, P < 0.001) and dermoid cysts (F = 22.01, P < 0.001) compared with women without peritoneal lesions. Higher FOXP3(+) Treg cell numbers in pathological lesions corresponded with significantly higher TGF-beta (P < 0.001) and lower IL-6 (P = 0.020) levels in peritoneal fluid of women with peritoneal lesions compared with women without lesions.Conclusions: This study confirms current speculation that endometriosis is related to alteration in Treg cells, causing survival and implantation of ectopic endometrial lesions in women with endometrioma and dermoid cysts. The findings may clarify why only 10% of women in the general population develop endometriosis despite cyclic menstruation with retrograde flow occurring in >90% of women.