Comparative Analysis of Microorganism Species Succession on Three Implant Surfaces With Different Roughness: An In Vivo Study
Comparative Analysis of Microorganism Species Succession on Three Implant Surfaces With Different Roughness: An In Vivo Study
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DOI：
10.1097/id.0b013e31820fb99e
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发表时间：
2011-04-01
影响因子：
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通讯作者：
Vidigal, Guaracilei Maciel, Jr.
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文献类型：
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作者：
de Freitas, Marcos Motta;Pereira da Silva, Cristiano Henrique;Vidigal, Guaracilei Maciel, Jr.
目的：由于几乎所有种植体系统都显示边缘骨丢失，并且一些种植体可能受到种植体周围炎的影响，因此为了实现有效的维持治疗，了解不同种植体表面上的生物膜发展非常重要。本人类研究的目的是比较分析3 surfaces.Materials和方法的微生物物种演替的动力学：三个商业纯钛片具有不同的粗糙度随机固定两侧的舌侧区域的Hawley型板的6名志愿者。参与者在以下实验阶段使用这些装置：1、3、7、14和21天，并被指示仅在口腔卫生程序期间移除丙烯酸板。生物膜的样品收获从每个片的中心区域，和24种细菌的存在下，确定使用生物标记板DNA-DNA Hybridization method.Results：结果显示没有统计学上的显着差异，在不同的表面上的细菌物种演替的动力学，在所有的时间段。粗糙表面并不比光滑表面更容易被致病菌定植。结论：表面粗糙度并不影响生物膜中细菌的物种演替。在研究的所有阶段，物种的演替是相似的。（种植体凹痕2011; 20：e14-e23）
Purpose: Because almost all implant systems reveal marginal bone loss and some implants can be affected by periimplantitis, to realize an effective maintenance treatment it is important to understand the biofilm development over different implant surfaces. The objective of this human study is to comparatively analyze the kinetics of microorganism species succession on 3 surfaces.Materials and Methods: Three commercially pure titanium sheets with different roughness were randomly fixed bilaterally on the lingual regions of Hawley-type plates of 6 volunteers. The participants used the apparatuses in the following experimental periods: 1, 3, 7, 14, and 21 days, and were instructed to remove the acrylic plates only during oral hygiene procedures. Samples of biofilm were harvested from the central areas of each sheet, and the presence of 24 bacterial species was identified using Checkerboard DNA-DNA Hybridization method.Results: The results showed no statistically significant differences with regard to the kinetics of bacterial species succession on the different surfaces, in all periods of time. Rough surfaces were not more prone to colonization by pathogenic bacteria than the smooth surfaces.Conclusion: The surface roughness does not affect the bacterial species succession in the biofilm. The succession of species was similar during all periods of the study. (Implant Dent 2011; 20:e14-e23)