Proteomic analysis of the bacterial cell cycle
Proteomic analysis of the bacterial cell cycle
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DOI：
10.1073/pnas.071538098
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发表时间：
2001-04-10
影响因子：
11.1
通讯作者：
Jenal, U
中科院分区：
文献类型：
--
作者：
Grünenfelder, B;Rummel, G;Jenal, U
使用全局方法来分析新月柄杆菌细胞周期期间的蛋白质合成和稳定性。大约四分之一 (979) 的估计 C. crescentus 基因产物是通过二维凝胶电泳检测到的，其中 144 个显示出差异的细胞周期表达模式。其中八十一种蛋白质通过质谱法鉴定出来，并被分配到各种功能组。模式分析表明共表达组在功能上聚集。在一个细胞周期中共有48种蛋白质被快速降解。研究还发现，超过一半的不稳定蛋白质是以细胞周期依赖性方式合成的，从而在快速蛋白质周转和细菌细胞周期的周期性之间建立了很强的相关性。据我们所知，这是蛋白水解在细菌细胞周期控制中发挥全局作用的第一个证据。
A global approach was used to analyze protein synthesis and stability during the cell cycle of the bacterium Caulobacter crescentus. Approximately one-fourth (979) of the estimated C. crescentus gene products were detected by two-dimensional gel electrophoresis, 144 of which showed differential cell cycle expression patterns. Eighty-one of these proteins were identified by mass spectrometry and were assigned to a wide variety of functional groups. Pattern analysis revealed that coexpression groups were functionally clustered. A total of 48 proteins were rapidly degraded in the course of one cell cycle. More than half of these unstable proteins were also found to be synthesized in a cell cycle-dependent manner, establishing a strong correlation between rapid protein turnover and the periodicity of the bacterial cell cycle. This is, to our knowledge, the first evidence for a global role of proteolysis in bacterial cell cycle control.