Surface plasmon resonance sensor for antibiotics detection based on photo-initiated polymerization molecularly imprinted array
Surface plasmon resonance sensor for antibiotics detection based on photo-initiated polymerization molecularly imprinted array
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DOI：
10.1016/j.talanta.2016.09.049
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发表时间：
2016-12-01
期刊：
影响因子：
6.1
通讯作者：
Wu, Jianmin
中科院分区：
文献类型：
--
作者：
Luo, Qiaohui;Yu, Neng;Wu, Jianmin
以纳米级分子印迹聚合物（MIP）膜为识别元件，研制了一种表面等离子体共振（SPR）传感器，用于抗生素环丙沙星（CIP）的选择性检测。采用原位光引发聚合法制备了SPR传感器芯片上的MIP膜，该方法具有聚合时间短、膜厚可控、均匀性好等优点。采用静态接触角测量和探针式轮廓仪对SPR传感器芯片表面MIP膜的润湿性和厚度进行了表征。该传感器对环丙沙星具有较高的选择性、灵敏度和稳定性。该传感器对环丙沙星及其结构类似物氧氟沙星的印迹因子分别为2.63和3.80，远高于对阿奇霉素、多巴胺和青霉素的印迹因子。在10（-11）-10（-7）mol L-1范围内，表面等离子体共振响应与CIP浓度呈良好的线性关系。该传感器在循环检测过程中也表现出良好的重复性和稳定性。在光引发聚合法的基础上，制备了表面等离子体共振成像（SPRi）芯片。MIPs-SPRi传感器对环丙沙星和阿奇霉素显示出不同的响应模式，揭示了识别不同抗生素分子的能力。
A surface plasmon resonance (SPR) sensor combined with nanoscale molecularly imprinted polymer (MIP) film as recognition element was developed for selective detection of the antibiotic ciprofloxacin (CIP). The MIP film on SPR sensor chip was prepared by in situ photo-initiated polymerization method which has the advantages of short polymerization time, controllable thickness and good uniformity. The surface wettability and thickness of MIP film on SPR sensor chip were characterized by static contact angle measurement and stylus profiler. The MIP-SPR sensor exhibited high selectivity, sensitivity and good stability for ciprofloxacin. The imprinting factors of the MIP-SPR sensor to ciprofloxacin and its structural analogue ofloxacin were 2.63 and 3.80, which is much higher than those to azithromycin, dopamine and penicillin. The SPR response had good linear relation with CIP concentration over the range 10(-11)-10(-7) mol L-1. The MIP-SPR sensor also showed good repeatability and stability during cyclic detections. On the basis of the photo-initiated polymerization method, a surface plasmon resonance imaging (SPRi) chip modified with three types of MIP sensing spots was fabricated. The MIPs-SPRi sensor shows different response patterns to ciprofloxacin and azithromycin, revealing the ability to recognize different antibiotic molecules.