Desert locusts Schistocerca gregaria (Acrididae: Orthoptera) do not lay eggs in old sand: Why?
Desert locusts Schistocerca gregaria (Acrididae: Orthoptera) do not lay eggs in old sand: Why?
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沙漠蝗虫 Schistocerca gregaria（蝗科：直翅目）不在旧沙中产卵：为什么？
DOI：
10.1007/s13355-017-0518-8
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发表时间：
2017
影响因子：
1.3
通讯作者：
Sugahara Ryohei
中科院分区：
文献类型：
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作者：
Tanaka Seiji;Sugahara Ryohei
有时候，Schistocerca gregarias拒绝在笼子里的“老沙子”里产卵。我们调查了它们为什么拒绝旧沙产卵。当新沙与旧沙同时出现时，蝗虫只在新沙中产卵，表明旧沙中含有产卵抑制因子。我们研究了这个因素是否来自蝗虫粪便或喂草（雀麦）。当蝗虫与含有粪便的沙子一起出现时，蝗虫将所有的卵产在有草的沙子中。相反，当同时提供干净的沙子时，很少有卵产到有草的沙子中，这表明OI因子来源于草，并集中在粪便中。与乙醇和丙酮相比，用水可以有效地从粪便中提取OI因子。通过用水提取粪便1 min检测OI活性，但提取时间越长活性越高。粪便水提液煮沸后仍保持OI活性。埋在含有粪便提取物的沙子中的卵没有孵化，而埋在干净的沙子或含有草提取物的沙子中的卵大多数孵化，表明OI活性和对卵的致死作用之间的相关性。
Schistocerca gregariasometimes refuse to lay eggs into the “old sand” that is kept in their cages for several days. We investigated why they rejected the old sand for oviposition. Locusts exclusively laid eggs into new sand when presented together with old sand, indicating that the old sand contained an oviposition-inhibiting (OI) factor. We examined whether this factor was derived from locust feces or fed grass (Bromus catharticus). Locusts laid all eggs into the sand with grass when presented together with sand containing feces. In contrast, few eggs were laid into the sand with grass when clean sand was offered at the same time, suggesting that the OI factor originated from the grass and was concentrated in the feces. The OI factor was efficiently extracted from feces with water compared with ethanol and acetone. OI activity was detected by extraction of feces with water for 1 min, although a longer extraction time yielded higher activity. The water extract of feces retained OI activity after boiling. None of the eggs which were buried in sand containing fecal extract hatched, whereas most of the eggs buried in clean sand or sand containing grass extract hatched, showing a correlation between OI activity and a lethal effect on eggs.