Non-recovery animal model of severe facial paralysis induced by freezing the facial canal
Non-recovery animal model of severe facial paralysis induced by freezing the facial canal
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DOI：
10.1016/j.anl.2020.03.012
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发表时间：
2020-10-01
影响因子：
1.7
通讯作者：
Hato, Naohito
中科院分区：
文献类型：
--
作者：
Joko, Tomonori;Yamada, Hiroyuki;Hato, Naohito
目的：周围性面神经麻痹的一些病例对治疗有抵抗力，因此，需要一个面神经麻痹的非恢复模型来开发新的治疗策略。方法：选用10只8周龄雌性Hartley豚鼠，体重400 ~ 500 g，建立重度面瘫动物模型。面神经管的垂直段通过耳泡进入，而不去除面神经管的骨性壁。然后使用冷冻喷雾将髓管冷冻5秒。面部运动，神经电图（ENoG），组织学，和温度的变化进行了evaluated.Results：所有的动物表现出完全面瘫后立即程序和逐渐恢复，但是，并不是所有的冷冻后15周完全恢复。所有动物（10/10）冷冻后1周的ENoG值为0%。冷冻后一周的组织学检查显示，大部分垂直放置的有髓神经纤维已被冷冻显着影响和变性。冷冻15周后，垂直排列的有髓神经纤维数量增多，但神经纤维较正常神经纤维小，形态扭曲。结论：冷冻面神经管可诱导Hartley豚鼠完全性面瘫。行为学、神经电图和组织病理学资料表明，面瘫严重且持续时间长。该模型可能有助于开发治疗重度面瘫的新方法，并促进面神经再生的基础研究。(C)2020日本耳鼻喉科学会Elsevier B. V.出版，保留所有权利。
Objective: Some cases of peripheral facial paralysis are resistant to treatment, thus, a nonrecovery model of facial paralysis is needed to develop new treatment strategies for this condition. The purpose of the current study was to develop an animal model of which facial palsy was severe and prolonged.Methods: Ten 8-week-old female Hartley guinea pigs weighing between 400 and 500 g were used for the animal model. The vertical segment of the facial canal was accessed via the otic bulla, without removing the bony wall of the facial canal. The canal was then frozen for 5 s using freeze spray. Facial movements, electroneurography (ENoG), histology, and changes in temperature were evaluated.Results: All animals exhibited complete facial paralysis immediately after the procedure and recovered gradually, however, not all of them had recovered completely 15 weeks after freezing. The ENoG values one week after freezing for all animals (10/10) were 0%. Histological examination one week after freezing revealed that most of the vertically placed myelinated nerve fibers which had been frozen were remarkably affected and denatured. The number of vertically placed myelinated nerve fibers increased 15 weeks after freezing, but the nerve fibers were smaller than normal nerve fibers and were distorted in shape.Conclusion: Complete facial paralysis was induced in Hartley guinea pigs by freezing the facial canal. The behavioral, ENoG, and histopathological data suggest that the facial paralysis was severe and prolonged. This model may assist in developing novel treatment for severe facial palsy and facilitate basic research on facial nerve regeneration. (C) 2020 Oto-Rhino-Laryngological Society of Japan Inc. Published by Elsevier B.V. All rights reserved.