Migratory fate and differentiation of blood monocyte subsets
Migratory fate and differentiation of blood monocyte subsets
复制标题
DOI：
10.1016/j.imbio.2006.05.025
复制
发表时间：
2006-01-01
期刊：
影响因子：
2.8
通讯作者：
Randolph, Gwendalyn J.
中科院分区：
文献类型：
--
作者：
Tacke, Frank;Randolph, Gwendalyn J.
单核细胞是组织巨噬细胞和树突状细胞 (DC) 的循环前体细胞。单核细胞衍生的巨噬细胞和树突状细胞在炎症过程中的先天性和适应性免疫中发挥着关键作用，并且人们相信单核细胞在稳态过程中也在外周组织中维持这些群体。然而，稳定状态下血液单核细胞对任何 DC 库的持续补充仍有待建立，并且一些巨噬细胞群可能在稳定状态下自我更新。最近对与人类单核细胞亚群非常相似的小鼠单核细胞亚群的鉴定启发了多种技术，其中可以在体内轻松追踪单核细胞以解决这些关键问题。有两种主要的单核细胞亚群，它们的趋化因子受体 (CCR) 和粘附分子表达以及迁移和分化特性各不相同。在人类中，“经典”CD14(+) CD16(-) 单核细胞表达 CCR2、CD64、CD62L，而“非经典”CD14(低) CD16(+) 单核细胞缺乏 CCR2。它们在小鼠中的对应物分别是 CCR2(+) Gr-1(hi) 和 CCR2(-) Gr-1(low) 单核细胞。 Gr-1(hi) (Ly6C(hi)) 单核细胞被募集到炎症部位，例如：发炎的皮肤或急性发炎的腹膜，并在炎症或传染病模型中产生巨噬细胞和 DC，并在皮肤炎症后产生表皮朗格汉斯细胞。 Gr-1（低）单核细胞已被提议作为稳态 DC 的前体，但实验证据尚有限。幸运的是，单核细胞命运研究的进展速度正在迅速加快，人们期望我们很快就能更多地了解单核细胞稳态和炎症的生物学特性。 (c) 2006 年爱思唯尔有限公司。版权所有。
Monocytes are established circulating precursors for tissue macrophages and dendritic cells (DCs). Monocyte-derived macrophages and DCs fulfill critical roles in innate and adaptive immunity during inflammation, and it is believed that monocytes also maintain these populations in peripheral tissues during homeostasis. However, the continuous replenishment of any DC pool by blood monocytes in the steady state remains to be established, and some macrophage populations may be self-renewing in the steady state. Recent identification of mouse monocyte subsets that closely resemble human monocyte subsets has inspired a variety of techniques wherein monocytes can be readily traced in vivo to address these critical questions. There are two major monocyte subsets that vary in chemokine receptor (CCR) and adhesion molecule expression, and in migratory and differentiation properties. In humans, 'classical' CD14(+) CD16(-) monocytes express CCR2, CD64, CD62L, whereas 'non-classical' CD14(low) CD16(+) monocytes lack CCR2. Their counterparts in mice are CCR2(+) Gr-1(hi) and CCR2(-) Gr-1(low) monocytes, respectively. Gr-1(hi) (Ly6C(hi)) monocytes are recruited to inflammatory sites, e.g. inflamed skin or acutely inflamed peritoneum and give rise to macrophages and DCs in inflammatory or infectious disease models and to epidermal Langerhans cells after skin inflammation. Gr-1(low) monocytes have been proposed as precursors for steady state DCs, but experimental evidence is as of yet limited. Fortunately, the rate of progress in the study of monocyte fate is rapidly picking up pace, giving rise to the expectation that we will soon know much more about the biology of monocytes in the steady state and inflammation. (c) 2006 Elsevier GmbH. All rights reserved.