In vivo tissue/biomaterials toxic responses: Correlation with cytotoxic potential but not cell attachment
In vivo tissue/biomaterials toxic responses: Correlation with cytotoxic potential but not cell attachment
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DOI：
10.1016/0267-6605(94)90085-x
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发表时间：
1994-01-01
期刊：
影响因子：
--
通讯作者：
Nakamura, Akitada
中科院分区：
文献类型：
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作者：
Tsuchiya, Toshie;Ikarashi, Yoshiaki;Nakamura, Akitada
使用镜片材料、辐射硫化天然橡胶乳胶（RVNRL）和标准参考材料（SRM）研究了细胞附着、细胞毒性和体内组织反应（例如肌肉炎症和眼睛刺激）之间的关系。五种 RVNRL 材料可诱导肌肉组织炎症，具有细胞毒性，并显着降低细胞附着活性。由分段聚氨酯制成的 SRM 的细胞毒性潜力与细胞附着活性的降低密切相关。然而，在镜片材料中，氟硅丙烯酸酯检测到了细胞毒性，而聚甲基丙烯酸甲酯(PMMA)则没有检测到细胞毒性，尽管PMMA的细胞附着活性最低。佩戴各种镜片引起的眼睛刺激与细胞毒性潜力相关（r = -0.90），但与细胞附着活动无关。总之，体内毒性组织反应与细胞毒性潜力密切相关，但并不总是与细胞附着活性相关。我们的实验结果还表明，直接接触集落形成主要受到细胞毒性的抑制，而不是细胞附着的低活性的抑制。
The relationships between cell attachment, cytotoxicity and in vivo tissue response such as muscle inflammation and eye irritation were investigated using lens materials, radiation vulcanized natural rubber latex (RVNRL) and standard reference materials (SRM). Five RVNRL materials, which induced muscle tissue inflammation, were cytotoxic and also significantly decreased cell attachment activities. The cytotoxic potential of a SRM made of segmented polyurethane correlated well with the decrease in cell attachment activity. However, among lens materials, cytotoxicity was detected in fluorosilicone acrylate but not in poly(methylmethacrylate) (PMMA), although the cell attachment activity of PMMA was the lowest. Eye irritation induced by wearing various kinds of lenses correlated with cytotoxic potential (r = -0.90) but not with cell attachment activities. In conclusion, in vivo toxic tissue responses correlated well with cytotoxic potentials but not always with cell attachment activity. Our experimental results also showed that direct contact colony formation was mainly inhibited by cytotoxicity, but not the low activity of cell attachment.