Facile fabrication of tissue-engineered constructs using nanopatterned cell sheets and magnetic levitation.
Facile fabrication of tissue-engineered constructs using nanopatterned cell sheets and magnetic levitation.
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DOI：
10.1088/1361-6528/aa55e0
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发表时间：
2017-02-17
期刊：
影响因子：
3.5
通讯作者：
Kim DH
中科院分区：
文献类型：
--
作者：
Penland N;Choi E;Perla M;Park J;Kim DH
我们报告了一种简单而通用的方法，通过将磁性细胞悬浮与基于热响应纳米制造基质（TNFS）的细胞片工程技术相结合，在体外制造具有预定细胞排列的无支架组织工程构建体。基于 TNFS 的纳米形貌为细胞排列的调节提供接触引导线索，并实现细胞片层转移，而磁性纳米粒子则促进细胞片层的磁悬浮。温度介导的热响应聚（N-异丙基丙烯酰胺）（PNIPAM）基质的表面润湿性变化使得细胞单层自发脱离，然后可以通过使用环形或盘形磁铁轻松操纵。我们开发的平台可以很容易地应用于生产包含复杂生理结构的组织工程结构，用于组织结构-功能关系、药物筛选和再生医学的研究。
We report a simple and versatile method for in vitro fabrication of scaffold-free tissue-engineered constructs with predetermined cellular alignment, by combining magnetic cell levitation with thermoresponsive nanofabricated substratum (TNFS) based cell sheet engineering technique. The TNFS based nanotopography provides contact guidance cues for regulation of cellular alignment and enables cell sheet transfer, while magnetic nanoparticles facilitate the magnetic levitation of the cell sheet. The temperature-mediated change in surface wettability of the thermoresponsive poly(N-isopropylacrylamide), PNIPAM, substratum enables the spontaneous detachment of cell monolayers, which can then be easily manipulated through use of a ring or disk shaped magnet. Our developed platform could be readily applicable to production of tissue-engineered constructs containing complex physiological structures for the study of tissue structure-function relationships, drug screening, and regenerative medicine.