Loss of c-kit expression in cultured melanoma cells.
Loss of c-kit expression in cultured melanoma cells.
复制标题
培养的黑色素瘤细胞中 c-kit 表达缺失。
作者：
N. Lassam;S. Bickford
原癌基因c-kit编码的受体酪氨酸激酶已被证明在黑素细胞发育中发挥关键作用。在这份报告中，我们问c-kit基因产物是否也参与促进转化黑素细胞的生长。我们发现，虽然c-Kit蛋白很容易在正常的人新生儿和成人黑素细胞中观察到，大多数从人黑色素瘤样品建立的细胞系不表达可检测水平的c-kit mRNA或蛋白。在正常和转化的小鼠黑素细胞中也观察到类似的差异表达模式。我们的研究结果提出了一种可能性，即c-kit基因表达的显着减少，促进或黑素细胞转化的结果。
The proto-oncogene c-kit encodes a receptor tyrosine kinase which has been shown to play a key role in melanocyte development. In this report we asked whether the c-kit gene product is also involved in promoting the growth of transformed melanocytes. We found that, while c-Kit protein was readily observed in normal human neonatal and adult melanocytes, the majority of cell lines established from human melanoma samples did not express detectable levels of c-kit mRNA or protein. A similar pattern of differential expression was also observed in normal and transformed murine melanocytes. Our findings raise the possibility that a marked reduction in c-kit gene expression either promotes or is a consequence of transformation in melanocytes.