RNAi and expression of retrotransposons MuERV-L and IAP in preimplantation mouse embryos
RNAi and expression of retrotransposons MuERV-L and IAP in preimplantation mouse embryos
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DOI：
10.1016/j.ydbio.2004.01.028
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发表时间：
2004-05-01
影响因子：
2.7
通讯作者：
Schultz, RM
中科院分区：
文献类型：
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作者：
Svoboda, P;Stein, P;Schultz, RM
在植入前小鼠胚胎基因组激活过程中，内源性逆转录病毒L(MERV-L)和脑池内A粒子(IAP)这两个自主的长末端重复序列反转录转座子均被激活，在2-细胞期和8-细胞期胚胎中均检测到正义和反义转录子。由于RNA干扰在小鼠着床前胚胎中发挥作用，我们通过抑制RNA干扰并检测这些转录本水平的相对变化来分析RNAi与小鼠传染性非典型肺炎病毒L和IAP表达的关系。我们通过向1-细胞胚胎注射mDICER siRNA或长mDICER dsRNA来抑制RNAi途径的起始步骤，并分析了MERV-L和IAP在8-细胞阶段的表达。这种方法导致靶向破坏mDICER mRNA，而不是HDAC1mRNA，抑制RNAi途径，并导致IAP和MERV-L转录丰度增加50%。这些结果表明，RNAi抑制了植入前胚胎中重复寄生序列的表达，从而有助于在有机体只由几个细胞组成的发育阶段保持基因组的完整性。(C)2004 Elsevier Inc.保留所有权利。
Both murine endogenous retrovirus-L (MuERV-L) and intracisternal A particle (IAP), two autonomous long terminal repeat (LTR) retrotransposons, are activated during genome activation in the preimplantation mouse embryo, and both sense and antisense transcripts are detected in 2-cell and 8-cell stage embryos. Because RNA interference (RNAi) functions in the preimplantation mouse embryo, we analyzed the relationship between RNAi and MuERV-L and IAP expression by inhibiting RNAi and measuring relative changes of the levels of these transcripts. We inhibited the initial step in the RNAi pathway by injecting 1-cell embryos with mDicer siRNA or long mDicer dsRNA and analyzed MuERV-L and IAP expression at the 8-cell stage. This approach resulted in the targeted destruction of mDicer mRNA, but not Hdac1 mRNA, inhibited the RNAi pathway, and resulted in a 50% increase in IAP and MuERV-L transcript abundance. These results suggest that RNAi constrains expression of repetitive parasitic sequences in preimplantation embryos, and thereby contributes to preserving genomic integrity at a stage of development when the organism consists of only a few cells. (C) 2004 Elsevier Inc. All rights reserved.