Functional Gene Expression Analysis of Tissue-Specific Isoforms of Mef2c
Functional Gene Expression Analysis of Tissue-Specific Isoforms of Mef2c
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DOI：
10.1007/s10571-011-9743-9
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发表时间：
2011
影响因子：
4
通讯作者：
Yoshiharu Sekiyama;H. Suzuki;T. Tsukahara
中科院分区：
文献类型：
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作者：
Yoshiharu Sekiyama;H. Suzuki;T. Tsukahara
mef2c包含三个可选外显子，在小鼠中产生六种mef2c异构体。Mef2cα1β亚型在神经元组织中表达，α2亚型在肌肉中表达，α1亚型在许多其他组织中表达。γ区包涵体和跳过的同工异构体在许多组织中以等量存在。在本研究中，研究了神经元细胞中Mef2c各组织特异性亚型转录活性的差异。使用mef2应答报告基因，在神经细胞以及其他先前描述的细胞系中发现了外显子β依赖的转激活。微阵列分析检测了每个Mef2c亚型的转录活性，并评估了不同亚型诱导的内源基因表达的差异。结果显示，mef2c异构体的过表达既依赖于异构体，也独立于异构体。
Mef2ccontains three alternative exons and generates sixMef2cisoforms in mice.Mef2cα1β isoforms are expressed in neuronal tissues, α2 isoforms are expressed in muscle, and α1 isoforms are expressed in many other tissues. The γ region inclusion and skipped isoforms are present in equal amounts in many tissues. In this study, differences in the transcriptional activities of each tissue-specific isoform of Mef2c in neuronal cells were examined. Using an MEF2-responsive reporter, exon β-dependent transactivation was found in neuronal cells, as well as in other cell lines previously described. Microarray analysis was used to examine the transcriptional activities of each Mef2c isoform and to assess differences in endogenous gene expression induced by the different isoforms. The results showed significant gene expression changes due to overexpression ofMef2cisoforms in both an isoforms-dependent and -independent manner.