Surface coat proteins of the pine wood nematode, Bursaphelenchus xylophilus: Profiles of stage- and isolate-specific characters
Surface coat proteins of the pine wood nematode, Bursaphelenchus xylophilus: Profiles of stage- and isolate-specific characters
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DOI：
10.1163/156854109x447006
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发表时间：
2009
期刊：
影响因子：
1.2
通讯作者：
R. Shinya;Y. Takeuchi;Natsuko Miura;K. Kuroda;M. Ueda;K. Futai
中科院分区：
文献类型：
--
作者：
R. Shinya;Y. Takeuchi;Natsuko Miura;K. Kuroda;M. Ueda;K. Futai
本研究是为了确定几种凝集素的表面涂层（SC）蛋白的结合模式的不同分离物和不同发育阶段的松材线虫（PWN），松材线虫。此外，SC蛋白质的详细特征进行了分析，通过使用分子技术。凝集素结合研究表明，SC与凝集素麦胚凝集素（WGA）的结合具有阶段特异性。WGA结合只观察到的第三阶段繁殖的青少年和蛋壳的外表面，这发生在有毒比无毒PWN菌株更频繁。与卵子结合的凝集素种类比与任何其他生命阶段结合的凝集素种类都多。为了表征，通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）分离提取的SC蛋白，并通过凝集素印迹法进行分析。结果表明，在线虫发育过程中，线虫SC的碳水化合物和蛋白质组成发生了变化。
The present study was made to determine the binding patterns of several lectins to the surface coat (SC) proteins of various isolates and developmental stages of the pine wood nematode (PWN), Bursaphelenchus xylophilus. Also, the detailed characteristics of the SC proteins were profiled by using molecular techniques. The lectin-binding study demonstrated the stage-specific characters of SC in binding to the lectin, wheat germ agglutinin (WGA). WGA binding was observed only to the outer surfaces of third-stage propagative juveniles and to the egg shells, and this occurred more frequently in virulent than in avirulent PWN isolates. A greater variety of lectins bound to eggs than to any other life stage. For characterisation, the SC proteins extracted were separated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and analysed by lectin blotting. The results showed that the carbohydrate and protein patterns of the SC of the PWN changed during nematode development.