Dual parameter flow cytometry studies in human lymphomas.
Dual parameter flow cytometry studies in human lymphomas.
复制标题
人类淋巴瘤的双参数流式细胞术研究。
DOI：
--
复制
发表时间：
1980
影响因子：
15.9
通讯作者：
R. Lukes
中科院分区：
文献类型：
--
作者：
S. Shackney;K. Skramstad;R. E. Cunningham;D. J. Dugas;T. Lincoln;R. Lukes
双参数流式细胞术的研究，使用库尔特体积和细胞DNA含量进行了单分散细胞悬液的64个样本的人淋巴瘤，慢性淋巴细胞白血病，毛细胞白血病，良性淋巴增生。淋巴瘤间平均Coulter体积的差异是由于平均G1细胞Coulter体积的内在差异以及较大S和G2细胞比例增加所致，尤其是在大B细胞淋巴瘤中。然而，大型非G1细胞对总体群体Coulter体积分布的相对贡献相对较小; S中细胞的存在不会使平均Coulter体积增加10%以上，即使在具有高S分数的样品中也是如此。在B细胞淋巴瘤中，平均G1细胞Coulter体积与S细胞分数的对数之间存在良好的相关性（r = 0.55）。有证据表明，在单个样品中，大细胞比小细胞增殖更快。这在每一个病例中都可以看到，无论是在正常样本中还是在淋巴瘤中，无论是在T细胞淋巴瘤还是在B细胞淋巴瘤中。在11例中，通过流式细胞术检测到非整倍体;在7例中，基于细胞库尔特体积差异，非整倍体细胞成分可以与二倍体细胞成分分开分析。在7例病例中的6例中，二倍体-非整倍体混合物的非整倍体组分的S分数高于二倍体组分（0.16 +/- 0.04 [SE] vs. 0.08 +/- 0.02）。这些发现被认为与淋巴瘤的组织病理学分类有关，并且与肿瘤的克隆选择和克隆进化的概念有关。
Dual parameter flow cytometry studies using Coulter volume and cell DNA content were carried out in monodisperse cell suspensions of 64 samples of human lymphoma, chronic lymphocytic leukemia, hairy cell leukemia, and benign lymphoid proliferations. Differences in mean Coulter volume among the lymphomas were due both to the intrinsic differences in mean G1 cell Coulter volume and to the presence of increased fractions of larger S and G2 cells, especially among the large B cell lymphomas. However, the relative contribution of large non-G1 cells to the overall population Coulter volume distribution was a relatively minor one; the presence of cells in S did not increase mean Coulter volume by more than 10%, even in samples with high S fractions. There was a good correlation between mean G1 cell Coulter volume and the log of the fraction of cells in S among the B cell lymphomas (r = 0.55). Evidence is presented that within individual samples, large cells proliferate more rapidly than small cells. This was seen in every case, both in the normal samples and in the lymphomas, and in the T cell lymphomas as well as in the B cell lymphomas. Aneuploidy was detected by flow cytometry in 11 cases; in 7 cases the aneuploid cell component could be analyzed separately from the diploid cell component on the basis of cell Coulter volume differences. The aneuploid components of diploid-aneuploid mixtures had higher S fractions than the diploid components in six of seven cases (0.16 +/- 0.04 [SE] vs. 0.08 +/- 0.02). These findings are considered in relation to the histopathological classification of the lymphomas, and in relation to the concept of clonal selection and clonal evolution of tumors.