Improvement of a CLE stochastic simulation of gene synthetic network with quorum sensing and feedback in a cell population
Improvement of a CLE stochastic simulation of gene synthetic network with quorum sensing and feedback in a cell population
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利用细胞群中的群体感应和反馈改进基因合成网络的 CLE 随机模拟
DOI：
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发表时间：
2015
期刊：
影响因子：
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通讯作者：
J. Picó
中科院分区：
文献类型：
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作者：
Y. Boada;A. Vignoni;J. L. Navarro;J. Picó
我们调查的可能性进行随机模拟的合成基因电路，其中包括细胞与细胞内反馈控制电路的细胞间通信系统。我们提出了一个实施方案的CLE随机模拟方法，使模拟基因合成电路涉及细胞到细胞的通信。我们找到最小数量的样本，确保人口的统计矩不退化。这使我们能够减少总的模拟时间，提高效率的方法。此外，我们发现，一个实现的人口互连的细胞，提供有足够的时间来执行时间平均，是有用的，以获得代表的长期时刻的人口。最后，我们通过分析LuxR启动子中的参数对群体噪声强度的影响，展示了改进方法的潜力。
We investigate the possibility of performing stochastic simulation of a synthetic gene circuit that includes a cell-to-cell communication system with an intracellular feedback control circuit. We propose an implementation of the CLE stochastic simulation method that makes possible to simulate gene synthetic circuits involving cell-to-cell communication. We find the minimum number of samples ensuring the statistic moments over the population do not degrade. This allows us to reduce the total simulation time and improve the efficiency of the method. Also we reveal that one realization of the population of interconnected cells, provided there is enough time to perform the time average, is useful to obtain representatives of the long-term moments of the population. Finally we show the potential of the improved approach by performing an analysis of the influence of the parameters in the LuxR promoter on the noise strength of the population.