Bioprocess development on microalgae-based CO2 fixation and bioethanol production using Scenedesmus obliquus CNW-N
Bioprocess development on microalgae-based CO2 fixation and bioethanol production using Scenedesmus obliquus CNW-N
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DOI：
10.1016/j.biortech.2013.02.119
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发表时间：
2013-10-01
影响因子：
11.4
通讯作者：
Chang, Jo-Shu
中科院分区：
文献类型：
--
作者：
Ho, Shih-Hsin;Li, Po-Jen;Chang, Jo-Shu
采用两阶段培养策略，利用本土微藻斜生栅藻 CNW-N 实现更高的二氧化碳固定和碳水化合物生产率，首先使用营养丰富的培养基培养以促进细胞生长，然后切换到营养缺乏的条件以引发碳水化合物积累。最佳生物量生产率、碳水化合物生产率和CO2固定率分别为681.4、352.9和1192.5 mg L-1 d(-1)，碳水化合物含量为51.8%（以干重计）。这一表现优于大多数相关研究的结果。微藻碳水化合物主要由葡萄糖组成，占总糖的近80%。用2% H2SO4 稀酸水解可以有效糖化湿微藻生物质，葡萄糖收率达到96-98%。以微藻酸性水解液为原料，采用单独水解发酵（SHF）工艺，乙醇浓度为8.55 g L-1，理论收率接近99.8%。 (C) 2013 Elsevier Ltd. 保留所有权利。
A two-stage cultivation strategy was applied to achieve greater CO2 fixation and carbohydrate productivity with an indigenous microalga Scenedesmus obliquus CNW-N, which was first cultivated using a nutrient-rich medium to promote cell growth, and was then switched to a nutrient-deficient condition to trigger carbohydrate accumulation. The optimal biomass productivity, carbohydrate productivity, and CO2 fixation rate were 681.4, 352.9, and 1192.5 mg L-1 d(-1), respectively, with a 51.8% carbohydrate content (based on dry weight). This performance is better than the results in most related studies. The microalgal carbohydrate was mainly composed of glucose, which accounts for nearly 80% of total sugars. Dilute acid hydrolysis with 2% H2SO4 can saccharify the wet microalgal biomass effectively, achieving a glucose yield of 96-98%. Using the acidic hydrolysate of the microalga as feedstock, the separate hydrolysis and fermentation (SHF) process gave an ethanol concentration of 8.55 g L-1, representing a theoretical yield of nearly 99.8%. (C) 2013 Elsevier Ltd. All rights reserved.