MinJ (YvjD) is a topological determinant of cell division in Bacillus subtilis
MinJ (YvjD) is a topological determinant of cell division in Bacillus subtilis
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DOI：
10.1111/j.1365-2958.2008.06469.x
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发表时间：
2008-12-01
影响因子：
3.6
通讯作者：
Kearns, Daniel B.
中科院分区：
文献类型：
--
作者：
Patrick, Joyce E.;Kearns, Daniel B.
在枯草芽孢杆菌中，FtsZ环的形成和细胞分裂是在细胞中间进行的，因为抑制蛋白MinC和MinD被蛋白DivIVA间接限制在细胞的两极。在这里，我们确定了MinJ，这是中间FtsZ定位的拓扑决定因素，充当divviva和MinD之间的中介。由于MinD活性不受限制，minJ突变的细胞在同源重组、群体运动和细胞分裂方面表现出多效性缺陷。MinJ通过直接的蛋白-蛋白相互作用将MinD定位到细胞极点，从而限制了MinD的活动。MinJ本身以依赖于DivIVA的方式定位到细胞极点。MinJ在其他低G+C革兰氏阳性细菌中保守，可能是这些生物细胞分裂位点选择的重要组成部分。
In Bacillus subtilis, FtsZ ring formation and cell division is favoured at the midcell because the inhibitor proteins MinC and MinD are indirectly restricted to the cell poles by the protein DivIVA. Here we identify MinJ, a topological determinant of medial FtsZ positioning that acts as an intermediary between DivIVA and MinD. Due to unrestricted MinD activity, cells mutated for minJ exhibited pleiotropic defects in homologous recombination, swarming motility and cell division. MinJ restricted MinD activity by localizing MinD to the cell poles through direct protein-protein interaction. MinJ itself localized to cell poles in a manner that was dependent on DivIVA. MinJ is conserved in other low G+C Gram-positive bacteria and may be an important component of cell division site selection in these organisms.