Fully three-dimensional defocus-gradient corrected backprojection in cryoelectron microscopy.
Fully three-dimensional defocus-gradient corrected backprojection in cryoelectron microscopy.
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DOI：
10.1016/j.ultramic.2010.04.002
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发表时间：
2010-08
期刊：
影响因子：
2.2
通讯作者：
Cernetic, Laslo
中科院分区：
文献类型：
--
作者：
Kazantsev, Ivan G.;Klukowska, Joanna;Herman, Gabor T.;Cernetic, Laslo
关键词：
詹森和Kornberg认识到显微镜景深是三维冷冻电子显微镜中一个重要的分辨率限制因素，提出了一种称为散焦梯度校正反投影（DGCBP）的概念，并通过计算机模拟说明DGCBP可以有效地消除景深限制。他们没有为他们的概念提供数学上的证明。我们的论文提供了这一点，通过显示（在无噪声数据可用于所有投影方向的理想情况下），从距离相关的模糊产生的数据的基础上DGCBP获得的重建基本上是相同的，通过一个经典的方法从其线积分的三维物体的重建。该方法足够通用，可适用于校正投影数据收集期间任何与距离相关的模糊。我们提出了一个新的实施DGCBP的概念，一个密切关注其理由的数学，并说明它使用的描述phantomy和他们的重建有限的距离依赖模糊的投影。
Recognizing that the microscope depth of field is a significant resolution-limiting factor in three-dimensional cryoelectron microscopy, Jensen and Kornberg proposed a concept they called defocus-gradient corrected backprojection (DGCBP) and illustrated by computer simulations that DGCBP can effectively eliminate the depth of field limitation. They did not provide a mathematical justification for their concept. Our paper provides this, by showing (in the idealized case of noiseless data being available for all projection directions) that the reconstructions obtained based on DGCBP from data produced with distance-dependent blurring are essentially the same as what is obtained by a classical method of reconstruction of a 3D object from its line integrals. The approach is general enough to be applicable for correcting for any distance-dependent blurring during projection data collection. We present a new implementation of the DGCBP concept, one that closely follows the mathematics of its justifications, and illustrate it using mathematically-described phantoms and their reconstructions from finitely-many distance-dependently blurred projections.
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影响因子：
2.2
作者：
Jensen, GJ;Kornberg, RD
通讯作者：
Kornberg, RD
影响因子：
1.7
作者：
Welling, Joel S.;Eddy, William F.;Young, Terence K.
通讯作者：
Young, Terence K.
影响因子：
2.2
作者：
DeRosier, DJ
通讯作者：
DeRosier, DJ
影响因子：
5.4
作者：
Eldar, Yonina C.;Unser, Michael
通讯作者：
Unser, Michael
影响因子：
2.1
作者：
DEFRISE, M
通讯作者：
DEFRISE, M