Ga14 in the Drosophila female germline
Ga14 in the Drosophila female germline
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DOI：
10.1016/s0925-4773(98)00157-9
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发表时间：
1998-11-01
影响因子：
2.6
通讯作者：
Rorth, P
中科院分区：
文献类型：
--
作者：
Rorth, P
模块化Ga14系统已被证明是果蝇条件基因表达的一个非常有用的工具。一个限制是该系统无法在女性生殖系中发挥作用。本文介绍了一种在卵子发生过程中起作用的改进的Ga14系统。为了实现种系表达，关键是改变ga14应答表达载体中的基础启动子和3'-UTR（产生UASp）。基础启动子和异源3'- utr通常被认为是中性的，但正如这里所示，可以赋予嵌合转录物定性的组织特异性。利用改进的Ga14系统研究果蝇FGF同源无枝FGF受体呼吸配体在边界细胞迁移中的作用。胚胎中FGF信号引导气管细胞迁移。然而，卵巢中无分枝基因的错误表达对边界细胞的迁移没有影响。因此，边缘细胞和气管细胞的引导方式不同。(C) 1998爱思唯尔科学爱尔兰有限公司版权所有。
The modular Ga14 system has proven to be an extremely useful tool for conditional gene expression in Drosophila. One limitation has been the inability of the system to work in the female germline. A modified Ga14 system that works throughout oogenesis is presented here. To achieve germline expression, it was critical to change the basal promoter and 3'-UTR in the Ga14-responsive expression vector (generating UASp). Basal promoters and heterologous 3'-UTRs are often considered neutral, but as shown here, can endow qualitative tissue-specificity to a chimeric transcript. The modified Ga14 system was used to investigate the role of the Drosophila FGF homologue branchless, ligand for the FGF receptor breathless, in border cell migration. FGF signaling guides tracheal cell migration in the embryo. However, misexpression of branchless in the ovary had no effect on border cell migration. Thus border cells and tracheal cells appear to be guided differently. (C) 1998 Elsevier Science Ireland Ltd. All rights reserved.